Gene:  SKIL, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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The protein encoded by this gene is a component of the SMAD pathway which regulates cell growth and differentiation through transforming growth factor-beta (TGFB). In the absence of ligand the encoded protein binds to the promoter region of TGFB-responsive genes and recruits a nuclear repressor complex. TGFB signaling causes SMAD3 to enter the nucleus and degrade this protein allowing these genes to be activated. Four transcript variants encoding three different isoforms have been found for this gene. [provided by RefSeq Oct 2011]

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PrimePCR™ SYBR® Green Assay: SKIL, Human
SKI-like oncogene

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0014721
Info:   Same primer pair as used in probe assay qHsaCIP0029527
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PrimePCR™ Probe Assay: SKIL, Human
SKI-like oncogene

Assay Type: Probe
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCIP0029527
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PrimePCR™ ddPCR™ Copy Number Assay: SKIL, Human
SKI-like oncogene

Assay Type: Probe
Application: Copy Number Variation
Unique Assay ID: dHsaCP2500464
Info:   66nt amplicon; exonic
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:SKIL p.T258A, Human
SKI-like oncogene

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2504758
Info:   69nt amplicon; exonic; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:SKIL WT for p.T258A, Human
SKI-like oncogene

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2504759
Info:   69nt amplicon; exonic; in-silico
 
Fluorophore:   HEX
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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