Gene:  SHMT2, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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This gene encodes the mitochondrial form of a pyridoxal phosphate-dependent enzyme that catalyzes the reversible reaction of serine and tetrahydrofolate to glycine and 510-methylene tetrahydrofolate. The encoded product is primarily responsible for glycine synthesis. The activity of the encoded protein has been suggested to be the primary source of intracellular glycine. The gene which encodes the cytosolic form of this enzyme is located on chromosome 17. Alternative splicing results in multiple transcript variants. [provided by RefSeq Oct 2009]

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PrimePCR™ SYBR® Green Assay: SHMT2, Human
serine hydroxymethyltransferase 2 (mitochondrial)

Assay Type: SYBR® Green
Assay Design: Exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0021707
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PrimePCR™ SYBR® Green Assay: SHMT2, Human
serine hydroxymethyltransferase 2 (mitochondrial)

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0047519
Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0054779
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PrimePCR™ Probe Assay: SHMT2, Human
serine hydroxymethyltransferase 2 (mitochondrial)

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0054779
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PrimePCR™ ddPCR™ Mutation Assay:SHMT2 p.R437H, Human
serine hydroxymethyltransferase 2 (mitochondrial)

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2501268
Info:   64nt amplicon; exonic; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:SHMT2 WT for p.R437H, Human
serine hydroxymethyltransferase 2 (mitochondrial)

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2501269
Info:   64nt amplicon; exonic; in-silico
 
Fluorophore:   HEX
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    Related pathways not available
Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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