Gene:  SHH, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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This gene encodes a protein that is instrumental in patterning the early embryo. It has been implicated as the key inductive signal in patterning of the ventral neural tube the anterior-posterior limb axis and the ventral somites. Of three human proteins showing sequence and functional similarity to the sonic hedgehog protein of Drosophila this protein is the most similar. The protein is made as a precursor that is autocatalytically cleaved; the N-terminal portion is soluble and contains the signalling activity while the C-terminal portion is involved in precursor processing. More importantly the C-terminal product covalently attaches a cholesterol moiety to the N-terminal product restricting the N-terminal product to the cell surface and preventing it from freely diffusing throughout the developing embryo. Defects in this protein or in its signalling pathway are a cause of holoprosencephaly (HPE) a disorder in which the developing forebrain fails to correctly separate into right and left hemispheres. HPE is manifested by facial deformities. It is also thought that mutations in this gene or in its signalling pathway may be responsible for VACTERL syndrome which is characterized by vertebral defects anal atresia tracheoesophageal fistula with esophageal atresia radial and renal dysplasia cardiac anomalies and limb abnormalities. Additionally mutations in a long range enhancer located approximately 1 megabase upstream of this gene disrupt limb patterning and can result in preaxial polydactyly. [provided by RefSeq Jul 2008]

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PrimePCR™ SYBR® Green Assay: SHH, Human
sonic hedgehog

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0037736
Info:   Same primer pair as used in probe assay qHsaCEP0040459
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PrimePCR™ Probe Assay: SHH, Human
sonic hedgehog

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0040459
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PrimePCR™ ddPCR™ Copy Number Assay: SHH, Human
sonic hedgehog

Assay Type: Probe
Application: Copy Number Variation
Unique Assay ID: dHsaCP1000055
 
Fluorophore:   FAM
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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