Gene:  SFRP1, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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This gene encodes a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. Members of this family act as soluble modulators of Wnt signaling; epigenetic silencing of SFRP genes leads to deregulated activation of the Wnt-pathway which is associated with cancer. This gene may also be involved in determining the polarity of photoreceptor cells in the retina. [provided by RefSeq Sep 2009]

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PrimePCR™ SYBR® Green Assay: SFRP1, Human
secreted frizzled-related protein 1

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0043005
Info:   Same primer pair as used in probe assay qHsaCEP0050267
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PrimePCR™ SYBR® Green Assay: SFRP1, Human
secreted frizzled-related protein 1

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0015548
Info:   Recommended - best coverage
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PrimePCR™ Probe Assay: SFRP1, Human
secreted frizzled-related protein 1

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0050267
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: SFRP1, Human
secreted frizzled-related protein 1

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5007833
Info:   EG; Same primer pair as used in qPCR assay qHsaCED0043005; exonic
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: SFRP1, Human
secreted frizzled-related protein 1

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5020913
Info:   EG; Same primer pair as used in qPCR assay qHsaCID0015548; Intron-spanning
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PrimePCR™ ddPCR™ Copy Number Assay: SFRP1, Human
secreted frizzled-related protein 1

Assay Type: Probe
Application: Copy Number Variation
Unique Assay ID: dHsaCP2500462
Info:   FAM; 70nt amplicon; exonic; FAM version of SFRP1 (dHsaCP2506562)
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Copy Number Assay: SFRP1, Human
secreted frizzled-related protein 1

Assay Type: Probe
Application: Copy Number Variation
Unique Assay ID: dHsaCP2506562
Info:   HEX; 70nt amplicon; exonic; HEX version of SFRP1 (dHsaCP2500462)
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Expression Probe Assay: SFRP1, Human
secreted frizzled-related protein 1

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5033978
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCEP0050267; exonic
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Expression Probe Assay: SFRP1, Human
secreted frizzled-related protein 1

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5033979
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCEP0050267; exonic
 
Fluorophore:   HEX
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    Related pathways not available
Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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