Gene:  SCN1A, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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Design and Validation of Real-Time PCR Primers
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The vertebrate sodium channel is a voltage-gated ion channel essential for the generation and propagation of action potentials mainly in nerve and muscle. Voltage-sensitive sodium channels are heteromeric complexes consisting of a large central pore-forming glycosylated alpha subunit and two smaller auxiliary beta subunits. This gene encodes the large alpha subunit and mutations in this gene have been associated with several epilepsy convulsion and migraine disorders. Alternative splicing results in multiple transcript variants. The RefSeq Project has decided to create four representative RefSeq records. Three of the transcript variants are supported by experimental evidence and the fourth contains alternate 5' untranslated exons the exact combination of which have not been experimentally confirmed for the full-length transcript.[provided by RefSeq Jan 2011]

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PrimePCR™ SYBR® Green Assay: SCN1A, Human
sodium channel, voltage-gated, type I, alpha subunit

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0009596
Info:   Same primer pair as used in probe assay qHsaCIP0027254
List Price:    $125.00
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PrimePCR™ Probe Assay: SCN1A, Human
sodium channel, voltage-gated, type I, alpha subunit

Assay Type: Probe
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCIP0027254
List Price:    $180.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: SCN1A, Human
sodium channel, voltage-gated, type I, alpha subunit

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5016501
Info:   EG; Same primer pair as used in qPCR assay qHsaCID0009596; Intron-spanning
List Price:    $125.00
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PrimePCR™ ddPCR™ Expression Probe Assay: SCN1A, Human
sodium channel, voltage-gated, type I, alpha subunit

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5048672
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCIP0027254; Intron-spanning
 
Fluorophore:   FAM
List Price:    $190.00
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PrimePCR™ ddPCR™ Expression Probe Assay: SCN1A, Human
sodium channel, voltage-gated, type I, alpha subunit

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5048673
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCIP0027254; Intron-spanning
 
Fluorophore:   HEX
List Price:    $190.00
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    Related pathways not available
Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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