Gene:  SCD, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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This gene encodes an enzyme involved in fatty acid biosynthesis primarily the synthesis of oleic acid. Transcripts of approximately 3.9 and 5.2 kb differing only by alternative polyadenlyation signals have been detected. A gene encoding a similar enzyme is located on chromosome 4 and a pseudogene of this gene is located on chromosome 17. [provided by RefSeq Feb 2012]

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PrimePCR™ SYBR® Green Assay: SCD, Human
stearoyl-CoA desaturase (delta-9-desaturase)

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0042705
Info:   Recommended - best efficiency; Same primer pair as used in probe assay qHsaCEP0049967
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PrimePCR™ SYBR® Green Assay: SCD, Human
stearoyl-CoA desaturase (delta-9-desaturase)

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0013037
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PrimePCR™ Probe Assay: SCD, Human
stearoyl-CoA desaturase (delta-9-desaturase)

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0049967
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: SCD, Human
stearoyl-CoA desaturase (delta-9-desaturase)

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5007556
Info:   EG; Same primer pair as used in qPCR assay qHsaCED0042705; exonic
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: SCD, Human
stearoyl-CoA desaturase (delta-9-desaturase)

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5019056
Info:   EG; Same primer pair as used in qPCR assay qHsaCID0013037; Intron-spanning
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PrimePCR™ ddPCR™ Expression Probe Assay: SCD, Human
stearoyl-CoA desaturase (delta-9-desaturase)

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5033424
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCEP0049967; exonic
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Expression Probe Assay: SCD, Human
stearoyl-CoA desaturase (delta-9-desaturase)

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5033425
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCEP0049967; exonic
 
Fluorophore:   HEX
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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