Gene:  RUNX2, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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This gene is a member of the RUNX family of transcription factors and encodes a nuclear protein with an Runt DNA-binding domain. This protein is essential for osteoblastic differentiation and skeletal morphogenesis and acts as a scaffold for nucleic acids and regulatory factors involved in skeletal gene expression. The protein can bind DNA both as a monomer or with more affinity as a subunit of a heterodimeric complex. Mutations in this gene have been associated with the bone development disorder cleidocranial dysplasia (CCD). Transcript variants that encode different protein isoforms result from the use of alternate promoters as well as alternate splicing. [provided by RefSeq Jul 2008]

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PrimePCR™ SYBR® Green Assay: RUNX2, Human
runt-related transcription factor 2

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0044067
Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0051329
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PrimePCR™ SYBR® Green Assay: RUNX2, Human
runt-related transcription factor 2

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0006726
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PrimePCR™ Probe Assay: RUNX2, Human
runt-related transcription factor 2

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0051329
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: RUNX2, Human
runt-related transcription factor 2

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5008761
Info:   EG; Same primer pair as used in qPCR assay qHsaCED0044067; exonic
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: RUNX2, Human
runt-related transcription factor 2

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5014672
Info:   EG; Same primer pair as used in qPCR assay qHsaCID0006726; Intron-spanning
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PrimePCR™ ddPCR™ Copy Number Assay: RUNX2, Human
runt-related transcription factor 2

Assay Type: Probe
Application: Copy Number Variation
Unique Assay ID: dHsaCP2506288
Info:   FAM; 67nt amplicon; exonic; FAM version of RUNX2 (dHsaCP2506434)
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Copy Number Assay: RUNX2, Human
runt-related transcription factor 2

Assay Type: Probe
Application: Copy Number Variation
Unique Assay ID: dHsaCP2506434
Info:   HEX; 67nt amplicon; exonic; HEX version of RUNX2 (dHsaCP2506288)
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Expression Probe Assay: RUNX2, Human
runt-related transcription factor 2

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5035834
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCEP0051329; exonic
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Expression Probe Assay: RUNX2, Human
runt-related transcription factor 2

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5035835
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCEP0051329; exonic
 
Fluorophore:   HEX
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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