Gene:  RPS6KB1, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates several residues of the S6 ribosomal protein. The kinase activity of this protein leads to an increase in protein synthesis and cell proliferation. Amplification of the region of DNA encoding this gene and overexpression of this kinase are seen in some breast cancer cell lines. Alternate translational start sites have been described and alternate transcriptional splice variants have been observed but have not been thoroughly characterized. [provided by RefSeq Jul 2008]

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PrimePCR™ SYBR® Green Assay: RPS6KB1, Human
ribosomal protein S6 kinase, 70kDa, polypeptide 1

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0043226
Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0050488
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PrimePCR™ SYBR® Green Assay: RPS6KB1, Human
ribosomal protein S6 kinase, 70kDa, polypeptide 1

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0015027
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PrimePCR™ Probe Assay: RPS6KB1, Human
ribosomal protein S6 kinase, 70kDa, polypeptide 1

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0050488
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PrimePCR™ ddPCR™ Copy Number Assay: RPS6KB1, Human
ribosomal protein S6 kinase, 70kDa, polypeptide 1

Assay Type: Probe
Application: Copy Number Variation
Unique Assay ID: dHsaCP1000054
 
Fluorophore:   FAM
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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