Gene:  RP11-155D18.11, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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This gene encodes a cytosolic homodimeric zinc-binding enzyme that catalyzes the hydrolysis of acylated L-amino acids to L-amino acids and an acyl group and has been postulated to function in the catabolism and salvage of acylated amino acids. This gene is located on chromosome 3p21.1 a region reduced to homozygosity in small-cell lung cancer (SCLC) and its expression has been reported to be reduced or undetectable in SCLC cell lines and tumors. The amino acid sequence of human aminoacylase-1 is highly homologous to the porcine counterpart and this enzyme is the first member of a new family of zinc-binding enzymes. Mutations in this gene cause aminoacylase-1 deficiency a metabolic disorder characterized by central nervous system defects and increased urinary excretion of N-acetylated amino acids. Alternative splicing of this gene results in multiple transcript variants. Read-through transcription also exists between this gene and the upstream ABHD14A (abhydrolase domain containing 14A) gene as represented in GeneID:100526760. A related pseudogene has been identified on chromosome 18. [provided by RefSeq Nov 2010]

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PrimePCR™ SYBR® Green Assay: RP11-155D18.11, Human
Aminoacylase-1

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0037071
Info:   Same primer pair as used in probe assay qHsaCEP0039794
List Price:    $130.00
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PrimePCR™ Probe Assay: RP11-155D18.11, Human
Aminoacylase-1

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0039794
List Price:    $190.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: RP11-155D18.11, Human
Aminoacylase-1

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5188305
Info:   EG; Same primer pair as used in qPCR assay qHsaCED0037071; exonic
List Price:    $130.00
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PrimePCR™ ddPCR™ Expression Probe Assay: RP11-155D18.11, Human
Aminoacylase-1

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5190301
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCEP0039794; exonic
 
Fluorophore:   FAM
List Price:    $195.00
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PrimePCR™ ddPCR™ Expression Probe Assay: RP11-155D18.11, Human
Aminoacylase-1

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5190302
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCEP0039794; exonic
 
Fluorophore:   HEX
List Price:    $195.00
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    Related pathways not available
Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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