Gene:  RET, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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This gene a member of the cadherin superfamily encodes one of the receptor tyrosine kinases which are cell-surface molecules that transduce signals for cell growth and differentiation. This gene plays a crucial role in neural crest development and it can undergo oncogenic activation in vivo and in vitro by cytogenetic rearrangement. Mutations in this gene are associated with the disorders multiple endocrine neoplasia type IIA multiple endocrine neoplasia type IIB Hirschsprung disease and medullary thyroid carcinoma. Two transcript variants encoding different isoforms have been found for this gene. Additional transcript variants have been described but their biological validity has not been confirmed. [provided by RefSeq Jul 2008]

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PrimePCR™ SYBR® Green Assay: RET, Human
ret proto-oncogene

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0013288
Info:   Same primer pair as used in probe assay qHsaCIP0028808
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PrimePCR™ Probe Assay: RET, Human
ret proto-oncogene

Assay Type: Probe
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCIP0028808
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PrimePCR™ ddPCR™ Copy Number Assay: RET, Human
ret proto-oncogene

Assay Type: Probe
Application: Copy Number Variation
Unique Assay ID: dHsaCP1000510
Info:   64nt amplicon; exonic
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:RET p.C634Y, Human
ret proto-oncogene

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2501046
Info:   65nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:RET WT for p.C634Y, Human
ret proto-oncogene

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2501047
Info:   65nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:RET p.E768D, Human
ret proto-oncogene

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2502508
Info:   65nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:RET WT for p.E768D, Human
ret proto-oncogene

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2502509
Info:   65nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:RET p.C630R, Human
ret proto-oncogene

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2503182
Info:   65nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:RET WT for p.C630R, Human
ret proto-oncogene

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2503183
Info:   65nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:RET p.C634R, Human
ret proto-oncogene

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2505168
Info:   65nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:RET WT for p.C634R, Human
ret proto-oncogene

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2505169
Info:   65nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   HEX
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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