Gene:  RB1, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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Design and Validation of Real-Time PCR Primers
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Control and Reference Assays for Real-Time PCR

The protein encoded by this gene is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB) bladder cancer and osteogenic sarcoma. [provided by RefSeq Jul 2008]

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PrimePCR™ SYBR® Green Assay: RB1, Human
retinoblastoma 1

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0044928
Info:   Same primer pair as used in probe assay qHsaCEP0052190
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PrimePCR™ SYBR® Green Assay: RB1, Human
retinoblastoma 1

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0007095
Info:   Recommended - best coverage
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PrimePCR™ Probe Assay: RB1, Human
retinoblastoma 1

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0052190
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PrimePCR™ ddPCR™ Copy Number Assay: RB1, Human
retinoblastoma 1

Assay Type: Probe
Application: Copy Number Variation
Unique Assay ID: dHsaCP1000052
 
Fluorophore:   FAM
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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