Gene:  RB1, Human

print
PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

Why PrimePCR?

PrimePCR
Lookup Tool
Design and Validation of Real-Time PCR Primers
Pathway Curation
Control and Reference Assays for Real-Time PCR

The protein encoded by this gene is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB) bladder cancer and osteogenic sarcoma. [provided by RefSeq Jul 2008]

Select a product type :   All   qPCR   ddPCR
PrimePCR™ SYBR® Green Assay: RB1, Human
retinoblastoma 1

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0044928
Info:   Same primer pair as used in probe assay qHsaCEP0052190
List Price:   
Your Price:   Log In
Quantity:  
 
Add to Custom Plate
PrimePCR™ SYBR® Green Assay: RB1, Human
retinoblastoma 1

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0007095
Info:   Recommended - best coverage
List Price:   
Your Price:   Log In
Quantity:  
 
Add to Custom Plate
PrimePCR™ Probe Assay: RB1, Human
retinoblastoma 1

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0052190
List Price:   
Your Price:   Log In
Quantity:  
 
PrimePCR™ ddPCR™ Copy Number Assay: RB1, Human
retinoblastoma 1

Assay Type: Probe
Application: Copy Number Variation
Unique Assay ID: dHsaCP1000052
Info:   117nt amplicon; crosses exon-intron junction
 
Fluorophore:   FAM
List Price:   
Your Price:   Log In
Quantity:  
 
PrimePCR™ ddPCR™ Copy Number Assay: RB1, Human
retinoblastoma 1

Assay Type: Probe
Application: Copy Number Variation
Unique Assay ID: dHsaCP2500456
Info:   69nt amplicon; exonic
 
Fluorophore:   FAM
List Price:   
Your Price:   Log In
Quantity:  
 
PrimePCR™ ddPCR™ Mutation Assay:RB1 p.R320*, Human
retinoblastoma 1

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2500756
Info:   100nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   FAM
List Price:   
Your Price:   Log In
Quantity:  
 
PrimePCR™ ddPCR™ Mutation Assay:RB1 WT for p.R320*, Human
retinoblastoma 1

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2500757
Info:   100nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   HEX
List Price:   
Your Price:   Log In
Quantity:  
 
PrimePCR™ ddPCR™ Mutation Assay:RB1 p.S82P, Human
retinoblastoma 1

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2501052
Info:   76nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   FAM
List Price:   
Your Price:   Log In
Quantity:  
 
PrimePCR™ ddPCR™ Mutation Assay:RB1 WT for p.S82P, Human
retinoblastoma 1

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2501053
Info:   76nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   HEX
List Price:   
Your Price:   Log In
Quantity:  
 
PrimePCR™ ddPCR™ Mutation Assay:RB1 p.R358*, Human
retinoblastoma 1

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2501212
Info:   80nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   FAM
List Price:   
Your Price:   Log In
Quantity:  
 
PrimePCR™ ddPCR™ Mutation Assay:RB1 WT for p.R358*, Human
retinoblastoma 1

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2501213
Info:   80nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   HEX
List Price:   
Your Price:   Log In
Quantity:  
 
PrimePCR™ ddPCR™ Mutation Assay:RB1 p.R556*, Human
retinoblastoma 1

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2501720
Info:   70nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   FAM
List Price:   
Your Price:   Log In
Quantity:  
 
PrimePCR™ ddPCR™ Mutation Assay:RB1 WT for p.R556*, Human
retinoblastoma 1

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2501721
Info:   70nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   HEX
List Price:   
Your Price:   Log In
Quantity:  
 
PrimePCR™ ddPCR™ Mutation Assay:RB1 p.R445*, Human
retinoblastoma 1

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2501838
Info:   69nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   FAM
List Price:   
Your Price:   Log In
Quantity:  
 
PrimePCR™ ddPCR™ Mutation Assay:RB1 WT for p.R445*, Human
retinoblastoma 1

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2501839
Info:   69nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   HEX
List Price:   
Your Price:   Log In
Quantity:  
 
PrimePCR™ ddPCR™ Mutation Assay:RB1 p.R787*, Human
retinoblastoma 1

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2501980
Info:   70nt amplicon; exonic; in-silico
 
Fluorophore:   FAM
List Price:   
Your Price:   Log In
Quantity:  
 
PrimePCR™ ddPCR™ Mutation Assay:RB1 WT for p.R787*, Human
retinoblastoma 1

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2501981
Info:   70nt amplicon; exonic; in-silico
 
Fluorophore:   HEX
List Price:   
Your Price:   Log In
Quantity:  
 
PrimePCR™ ddPCR™ Mutation Assay:RB1 p.R661W, Human
retinoblastoma 1

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2502390
Info:   64nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   FAM
List Price:   
Your Price:   Log In
Quantity:  
 
PrimePCR™ ddPCR™ Mutation Assay:RB1 WT for p.R661W, Human
retinoblastoma 1

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2502391
Info:   64nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   HEX
List Price:   
Your Price:   Log In
Quantity:  
 
PrimePCR™ ddPCR™ Mutation Assay:RB1 p.R579*, Human
retinoblastoma 1

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2503050
Info:   77nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   FAM
List Price:   
Your Price:   Log In
Quantity:  
 
PrimePCR™ ddPCR™ Mutation Assay:RB1 WT for p.R579*, Human
retinoblastoma 1

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2503051
Info:   77nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   HEX
List Price:   
Your Price:   Log In
Quantity:  
 
PrimePCR™ ddPCR™ Mutation Assay:RB1 p.Q685*, Human
retinoblastoma 1

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2503148
Info:   77nt amplicon; exonic; in-silico
 
Fluorophore:   FAM
List Price:   
Your Price:   Log In
Quantity:  
 
PrimePCR™ ddPCR™ Mutation Assay:RB1 WT for p.Q685*, Human
retinoblastoma 1

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2503149
Info:   77nt amplicon; exonic; in-silico
 
Fluorophore:   HEX
List Price:   
Your Price:   Log In
Quantity:  
 
PrimePCR™ ddPCR™ Mutation Assay:RB1 p.R251*, Human
retinoblastoma 1

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2503604
Info:   78nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   FAM
List Price:   
Your Price:   Log In
Quantity:  
 
PrimePCR™ ddPCR™ Mutation Assay:RB1 WT for p.R251*, Human
retinoblastoma 1

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2503605
Info:   78nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   HEX
List Price:   
Your Price:   Log In
Quantity:  
 
PrimePCR™ ddPCR™ Mutation Assay:RB1 p.D718G, Human
retinoblastoma 1

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2503734
Info:   88nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   FAM
List Price:   
Your Price:   Log In
Quantity:  
 
PrimePCR™ ddPCR™ Mutation Assay:RB1 WT for p.D718G, Human
retinoblastoma 1

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2503735
Info:   88nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   HEX
List Price:   
Your Price:   Log In
Quantity:  
 
PrimePCR™ ddPCR™ Mutation Assay:RB1 p.R552*, Human
retinoblastoma 1

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2503792
Info:   70nt amplicon; exonic; in-silico
 
Fluorophore:   FAM
List Price:   
Your Price:   Log In
Quantity:  
 
PrimePCR™ ddPCR™ Mutation Assay:RB1 WT for p.R552*, Human
retinoblastoma 1

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2503793
Info:   70nt amplicon; exonic; in-silico
 
Fluorophore:   HEX
List Price:   
Your Price:   Log In
Quantity:  
 
PrimePCR™ ddPCR™ Mutation Assay:RB1 p.E137*, Human
retinoblastoma 1

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2504050
Info:   84nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   FAM
List Price:   
Your Price:   Log In
Quantity:  
 
PrimePCR™ ddPCR™ Mutation Assay:RB1 WT for p.E137*, Human
retinoblastoma 1

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2504051
Info:   84nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   HEX
List Price:   
Your Price:   Log In
Quantity:  
 
PrimePCR™ ddPCR™ Mutation Assay:RB1 p.E440*, Human
retinoblastoma 1

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2504976
Info:   77nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   FAM
List Price:   
Your Price:   Log In
Quantity:  
 
PrimePCR™ ddPCR™ Mutation Assay:RB1 WT for p.E440*, Human
retinoblastoma 1

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2504977
Info:   77nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   HEX
List Price:   
Your Price:   Log In
Quantity:  
 
PrimePCR™ ddPCR™ Mutation Assay:RB1 p.E440K, Human
retinoblastoma 1

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2505174
Info:   77nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   FAM
List Price:   
Your Price:   Log In
Quantity:  
 
PrimePCR™ ddPCR™ Mutation Assay:RB1 WT for p.E440K, Human
retinoblastoma 1

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2505175
Info:   77nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   HEX
List Price:   
Your Price:   Log In
Quantity:  
 
PrimePCR™ ddPCR™ Mutation Assay:RB1 p.Q217*, Human
retinoblastoma 1

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2505524
Info:   80nt amplicon; exonic; in-silico
 
Fluorophore:   FAM
List Price:   
Your Price:   Log In
Quantity:  
 
PrimePCR™ ddPCR™ Mutation Assay:RB1 WT for p.Q217*, Human
retinoblastoma 1

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2505525
Info:   80nt amplicon; exonic; in-silico
 
Fluorophore:   HEX
List Price:   
Your Price:   Log In
Quantity:  
 
PrimePCR™ ddPCR™ Mutation Assay:RB1 p.C706C, Human
retinoblastoma 1

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2506162
Info:   80nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   FAM
List Price:   
Your Price:   Log In
Quantity:  
 
PrimePCR™ ddPCR™ Mutation Assay:RB1 WT for p.C706C, Human
retinoblastoma 1

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2506163
Info:   80nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   HEX
List Price:   
Your Price:   Log In
Quantity:  
 
Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
Get Help

Call us at 1-800-4-BIORAD (1-800-424-6723)

Sign Up for Bio-Rad Updates!
Enter your email address below to receive your choice of the latest news, promotions, and more.