Gene:  RASA1, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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The protein encoded by this gene is located in the cytoplasm and is part of the GAP1 family of GTPase-activating proteins. The gene product stimulates the GTPase activity of normal RAS p21 but not its oncogenic counterpart. Acting as a suppressor of RAS function the protein enhances the weak intrinsic GTPase activity of RAS proteins resulting in the inactive GDP-bound form of RAS thereby allowing control of cellular proliferation and differentiation. Mutations leading to changes in the binding sites of either protein are associated with basal cell carcinomas. Alternative splicing results in two isoforms where the shorter isoform lacking the N-terminal hydrophobic region but retaining the same activity appears to be abundantly expressed in placental but not adult tissues. [provided by RefSeq Jul 2008]

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PrimePCR™ SYBR® Green Assay: RASA1, Human
RAS p21 protein activator (GTPase activating protein) 1

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0017106
Info:   Same primer pair as used in probe assay qHsaCIP0031027
List Price:    $125.00
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PrimePCR™ Probe Assay: RASA1, Human
RAS p21 protein activator (GTPase activating protein) 1

Assay Type: Probe
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCIP0031027
List Price:    $180.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: RASA1, Human
RAS p21 protein activator (GTPase activating protein) 1

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5022253
Info:   EG; Same primer pair as used in qPCR assay qHsaCID0017106; Intron-spanning
List Price:    $125.00
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PrimePCR™ ddPCR™ Expression Probe Assay: RASA1, Human
RAS p21 protein activator (GTPase activating protein) 1

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5055296
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCIP0031027; Intron-spanning
 
Fluorophore:   FAM
List Price:    $190.00
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PrimePCR™ ddPCR™ Expression Probe Assay: RASA1, Human
RAS p21 protein activator (GTPase activating protein) 1

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5055297
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCIP0031027; Intron-spanning
 
Fluorophore:   HEX
List Price:    $190.00
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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