Gene:  RAG2, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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This gene encodes a protein that is involved in the initiation of V(D)J recombination during B and T cell development. This protein forms a complex with the product of the adjacent recombination activating gene 1 and this complex can form double-strand breaks by cleaving DNA at conserved recombination signal sequences. The recombination activating gene 1 component is thought to contain most of the catalytic activity while the N-terminal of the recombination activating gene 2 component is thought to form a six-bladed propeller in the active core that serves as a binding scaffold for the tight association of the complex with DNA. A C-terminal plant homeodomain finger-like motif in this protein is necessary for interactions with chromatin components specifically with histone H3 that is trimethylated at lysine 4. Mutations in this gene cause Omenn syndrome a form of severe combined immunodeficiency associated with autoimmune-like symptoms. [provided by RefSeq Jul 2008]

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PrimePCR™ SYBR® Green Assay: RAG2, Human
recombination activating gene 2

Assay Type: SYBR® Green
Assay Design: Exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0003632
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PrimePCR™ SYBR® Green Assay: RAG2, Human
recombination activating gene 2

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0056600
Info:   Same primer pair as used in probe assay qHsaCEP0057705
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PrimePCR™ Probe Assay: RAG2, Human
recombination activating gene 2

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0057705
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PrimePCR™ ddPCR™ Mutation Assay:RAG2 p.C385C, Human
recombination activating gene 2

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2501538
Info:   67nt amplicon; exonic; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:RAG2 WT for p.C385C, Human
recombination activating gene 2

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2501539
Info:   67nt amplicon; exonic; in-silico designed
 
Fluorophore:   HEX
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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