Gene:  RAF1, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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This gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K) which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2 which in turn phosphorylate to activate the serine/threonine specific protein kinases ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle apoptosis cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2. [provided by RefSeq Jul 2008]

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PrimePCR™ SYBR® Green Assay: RAF1, Human
v-raf-1 murine leukemia viral oncogene homolog 1

Assay Type: SYBR® Green
Assay Design: Exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0001147
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PrimePCR™ SYBR® Green Assay: RAF1, Human
v-raf-1 murine leukemia viral oncogene homolog 1

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0044423
Info:   Same primer pair as used in probe assay qHsaCEP0051685
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PrimePCR™ Probe Assay: RAF1, Human
v-raf-1 murine leukemia viral oncogene homolog 1

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0051685
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PrimePCR™ ddPCR™ Copy Number Assay: RAF1, Human
v-raf-1 murine leukemia viral oncogene homolog 1

Assay Type: Probe
Application: Copy Number Variation
Unique Assay ID: dHsaCP1000461
 
Fluorophore:   FAM
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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