Gene:  RAD50, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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The protein encoded by this gene is highly similar to Saccharomyces cerevisiae Rad50 a protein involved in DNA double-strand break repair. This protein forms a complex with MRE11 and NBS1. The protein complex binds to DNA and displays numerous enzymatic activities that are required for nonhomologous joining of DNA ends. This protein cooperating with its partners is important for DNA double-strand break repair cell cycle checkpoint activation telomere maintenance and meiotic recombination. Knockout studies of the mouse homolog suggest this gene is essential for cell growth and viability. Mutations in this gene are the cause of Nijmegen breakage syndrome-like disorder.[provided by RefSeq Apr 2010]

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PrimePCR™ SYBR® Green Assay: RAD50, Human
RAD50 homolog (S. cerevisiae)

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0013265
Info:   Same primer pair as used in probe assay qHsaCIP0028799
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PrimePCR™ Probe Assay: RAD50, Human
RAD50 homolog (S. cerevisiae)

Assay Type: Probe
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCIP0028799
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: RAD50, Human
RAD50 homolog (S. cerevisiae)

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5019208
Info:   EG; Same primer pair as used in qPCR assay qHsaCID0013265; Intron-spanning
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PrimePCR™ ddPCR™ Expression Probe Assay: RAD50, Human
RAD50 homolog (S. cerevisiae)

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5051298
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCIP0028799; Intron-spanning
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Expression Probe Assay: RAD50, Human
RAD50 homolog (S. cerevisiae)

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5051299
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCIP0028799; Intron-spanning
 
Fluorophore:   HEX
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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