Gene:  RAD18, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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Design and Validation of Real-Time PCR Primers
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The protein encoded by this gene is highly similar to S. cerevisiae DNA damage repair protein Rad18. Yeast Rad18 functions through its interaction with Rad6 which is an ubiquitin-conjugating enzyme required for post-replication repair of damaged DNA. Similar to its yeast counterpart this protein is able to interact with the human homolog of yeast Rad6 protein through a conserved ring-finger motif. Mutation of this motif results in defective replication of UV-damaged DNA and hypersensitivity to multiple mutagens. [provided by RefSeq Jul 2008]

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PrimePCR™ SYBR® Green Assay: RAD18, Human
RAD18 homolog (S. cerevisiae)

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0007443
Info:   Same primer pair as used in probe assay qHsaCIP0026472
List Price:    $125.00
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PrimePCR™ Probe Assay: RAD18, Human
RAD18 homolog (S. cerevisiae)

Assay Type: Probe
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCIP0026472
List Price:    $180.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: RAD18, Human
RAD18 homolog (S. cerevisiae)

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5015092
Info:   EG; Same primer pair as used in qPCR assay qHsaCID0007443; Intron-spanning
List Price:    $125.00
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PrimePCR™ ddPCR™ Expression Probe Assay: RAD18, Human
RAD18 homolog (S. cerevisiae)

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5047306
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCIP0026472; Intron-spanning
 
Fluorophore:   FAM
List Price:    $190.00
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PrimePCR™ ddPCR™ Expression Probe Assay: RAD18, Human
RAD18 homolog (S. cerevisiae)

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5047307
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCIP0026472; Intron-spanning
 
Fluorophore:   HEX
List Price:    $190.00
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    Related pathways not available
Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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