Gene:  PYGL, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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This gene encodes a homodimeric protein that catalyses the cleavage of alpha-14-glucosidic bonds to release glucose-1-phosphate from liver glycogen stores. This protein switches from inactive phosphorylase B to active phosphorylase A by phosphorylation of serine residue 15. Activity of this enzyme is further regulated by multiple allosteric effectors and hormonal controls. Humans have three glycogen phosphorylase genes that encode distinct isozymes that are primarily expressed in liver brain and muscle respectively. The liver isozyme serves the glycemic demands of the body in general while the brain and muscle isozymes supply just those tissues. In glycogen storage disease type VI also known as Hers disease mutations in liver glycogen phosphorylase inhibit the conversion of glycogen to glucose and results in moderate hypoglycemia mild ketosis growth retardation and hepatomegaly. Alternative splicing results in multiple transcript variants encoding different isoforms.[provided by RefSeq Feb 2011]

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PrimePCR™ SYBR® Green Assay: PYGL, Human
phosphorylase, glycogen, liver

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0017715
Info:   Same primer pair as used in probe assay qHsaCIP0031423
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PrimePCR™ Probe Assay: PYGL, Human
phosphorylase, glycogen, liver

Assay Type: Probe
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCIP0031423
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: PYGL, Human
phosphorylase, glycogen, liver

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5189671
Info:   EG; Same primer pair as used in qPCR assay qHsaCID0017715; Intron-spanning
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PrimePCR™ ddPCR™ Expression Probe Assay: PYGL, Human
phosphorylase, glycogen, liver

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5192883
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCIP0031423; Intron-spanning
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Expression Probe Assay: PYGL, Human
phosphorylase, glycogen, liver

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5192884
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCIP0031423; Intron-spanning
 
Fluorophore:   HEX
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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