Gene:  PSMD2, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base which contains 6 ATPase subunits and 2 non-ATPase subunits and a lid which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome the immunoproteasome is the processing of class I MHC peptides. This gene encodes one of the non-ATPase subunits of the 19S regulator lid. In addition to participation in proteasome function this subunit may also participate in the TNF signalling pathway since it interacts with the tumor necrosis factor type 1 receptor. A pseudogene has been identified on chromosome 1. [provided by RefSeq Jul 2008]

PrimePCR™ SYBR® Green Assay: PSMD2, Human
proteasome (prosome, macropain) 26S subunit, non-ATPase, 2

Assay Type: SYBR® Green
Assay Design: Exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0001772
Info:   Recommended - best coverage
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PrimePCR™ SYBR® Green Assay: PSMD2, Human
proteasome (prosome, macropain) 26S subunit, non-ATPase, 2

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0047092
Info:   Same primer pair as used in probe assay qHsaCEP0054353
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PrimePCR™ Probe Assay: PSMD2, Human
proteasome (prosome, macropain) 26S subunit, non-ATPase, 2

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0054353
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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