Gene:  PRKG1, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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Mammals have three different isoforms of cyclic GMP-dependent protein kinase (Ialpha Ibeta and II). These PRKG isoforms act as key mediators of the nitric oxide/cGMP signaling pathway and are important components of many signal transduction processes in diverse cell types. This PRKG1 gene on human chromosome 10 encodes the soluble Ialpha and Ibeta isoforms of PRKG by alternative transcript splicing. A separate gene on human chromosome 4 PRKG2 encodes the membrane-bound PRKG isoform II. The PRKG1 proteins play a central role in regulating cardiovascular and neuronal functions in addition to relaxing smooth muscle tone preventing platelet aggregation and modulating cell growth. This gene is most strongly expressed in all types of smooth muscle platelets cerebellar Purkinje cells hippocampal neurons and the lateral amygdala. Isoforms Ialpha and Ibeta have identical cGMP-binding and catalytic domains but differ in their leucine/isoleucine zipper and autoinhibitory sequences and therefore differ in their dimerization substrates and kinase enzyme activity. [provided by RefSeq Sep 2011]

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PrimePCR™ SYBR® Green Assay: PRKG1, Human
protein kinase, cGMP-dependent, type I

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0047754
Info:   Same primer pair as used in probe assay qHsaCEP0055014
List Price:    $125.00
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PrimePCR™ SYBR® Green Assay: PRKG1, Human
protein kinase, cGMP-dependent, type I

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0009678
Info:   Recommended - best coverage
List Price:    $125.00
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PrimePCR™ Probe Assay: PRKG1, Human
protein kinase, cGMP-dependent, type I

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0055014
List Price:    $180.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: PRKG1, Human
protein kinase, cGMP-dependent, type I

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5012053
Info:   EG; Same primer pair as used in qPCR assay qHsaCED0047754; exonic
List Price:    $125.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: PRKG1, Human
protein kinase, cGMP-dependent, type I

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5016560
Info:   EG; Same primer pair as used in qPCR assay qHsaCID0009678; Intron-spanning
List Price:    $125.00
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PrimePCR™ ddPCR™ Expression Probe Assay: PRKG1, Human
protein kinase, cGMP-dependent, type I

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5042416
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCEP0055014; exonic
 
Fluorophore:   FAM
List Price:    $190.00
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PrimePCR™ ddPCR™ Expression Probe Assay: PRKG1, Human
protein kinase, cGMP-dependent, type I

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5042417
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCEP0055014; exonic
 
Fluorophore:   HEX
List Price:    $190.00
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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