Gene:  POU5F1, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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This gene encodes a transcription factor containing a POU homeodomain. This transcription factor plays a role in embryonic development especially during early embryogenesis and it is necessary for embryonic stem cell pluripotency. A translocation of this gene with the Ewing's sarcoma gene t(6;22)(p21;q12) has been linked to tumor formation. Alternative splicing as well as usage of alternative translation initiation codons results in multiple isoforms one of which initiates at a non-AUG (CUG) start codon. Related pseudogenes have been identified on chromosomes 1 3 8 10 and 12. [provided by RefSeq Mar 2010]

PrimePCR™ SYBR® Green Assay: POU5F1, Human
POU class 5 homeobox 1

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0038334
Info:   Same primer pair as used in probe assay qHsaCEP0041056
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PrimePCR™ Probe Assay: POU5F1, Human
POU class 5 homeobox 1

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0041056
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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