Gene:  PIM1, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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The protein encoded by this gene belongs to the Ser/Thr protein kinase family and PIM subfamily. This gene is expressed primarily in B-lymphoid and myeloid cell lines and is overexpressed in hematopoietic malignancies and in prostate cancer. It plays a role in signal transduction in blood cells contributing to both cell proliferation and survival and thus provides a selective advantage in tumorigenesis. Both the human and orthologous mouse genes have been reported to encode two isoforms (with preferential cellular localization) resulting from the use of alternative in-frame translation initiation codons the upstream non-AUG (CUG) and downstream AUG codons (PMIDs:16186805 1825810).[provided by RefSeq Aug 2011]

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PrimePCR™ SYBR® Green Assay: PIM1, Human
pim-1 oncogene

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0014577
Info:   Same primer pair as used in probe assay qHsaCIP0029454
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PrimePCR™ Probe Assay: PIM1, Human
pim-1 oncogene

Assay Type: Probe
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCIP0029454
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PrimePCR™ ddPCR™ Mutation Assay:PIM1 p.S97N, Human
pim-1 oncogene

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2502882
Info:   65nt amplicon; exonic; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:PIM1 WT for p.S97N, Human
pim-1 oncogene

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2502883
Info:   65nt amplicon; exonic; in-silico designed
 
Fluorophore:   HEX
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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