Gene:  PIK3CB, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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This gene encodes an isoform of the catalytic subunit of phosphoinositide 3-kinase (PI3K). These kinases are important in signaling pathways involving receptors on the outer membrane of eukaryotic cells and are named for their catalytic subunit. The encoded protein is the catalytic subunit for PI3Kbeta (PI3KB). PI3KB has been shown to be part of the activation pathway in neutrophils which have bound immune complexes at sites of injury or infection. Alternative splicing results in multiple transcript variants. [provided by RefSeq Dec 2011]

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PrimePCR™ SYBR® Green Assay: PIK3CB, Human
phosphoinositide-3-kinase, catalytic, beta polypeptide

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0056640
Info:   Same primer pair as used in probe assay qHsaCEP0057745
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PrimePCR™ SYBR® Green Assay: PIK3CB, Human
phosphoinositide-3-kinase, catalytic, beta polypeptide

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0007171
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PrimePCR™ Probe Assay: PIK3CB, Human
phosphoinositide-3-kinase, catalytic, beta polypeptide

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0057745
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PrimePCR™ ddPCR™ Mutation Assay:PIK3CB p.L832W, Human
phosphoinositide-3-kinase, catalytic, beta polypeptide

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2506102
Info:   96nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:PIK3CB WT for p.L832W, Human
phosphoinositide-3-kinase, catalytic, beta polypeptide

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2506103
Info:   96nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   HEX
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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