Gene:  PHB, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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Prohibitin is an evolutionarily conserved gene that is ubiquitously expressed. It is thought to be a negative regulator of cell proliferation and may be a tumor suppressor. Mutations in PHB have been linked to sporadic breast cancer. Prohibitin is expressed as two transcripts with varying lengths of 3' untranslated region. The longer transcript is present at higher levels in proliferating tissues and cells suggesting that this longer 3' untranslated region may function as a trans-acting regulatory RNA. [provided by RefSeq Jul 2008]

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PrimePCR™ SYBR® Green Assay: PHB, Human
prohibitin

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0038133
Info:   Same primer pair as used in probe assay qHsaCIP0040856
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PrimePCR™ Probe Assay: PHB, Human
prohibitin

Assay Type: Probe
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCIP0040856
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: PHB, Human
prohibitin

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5025198
Info:   EG; Same primer pair as used in qPCR assay qHsaCID0038133; Intron-spanning
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PrimePCR™ ddPCR™ Expression Probe Assay: PHB, Human
prohibitin

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5059082
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCIP0040856; Intron-spanning
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Expression Probe Assay: PHB, Human
prohibitin

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5059083
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCIP0040856; Intron-spanning
 
Fluorophore:   HEX
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    Related pathways not available
Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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