Gene:  PDGFRA, Human

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This gene encodes a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer composed of both platelet-derived growth factor receptor alpha and beta polypeptides. Studies suggest that this gene plays a role in organ development wound healing and tumor progression. Mutations in this gene have been associated with idiopathic hypereosinophilic syndrome somatic and familial gastrointestinal stromal tumors and a variety of other cancers. [provided by RefSeq Mar 2012]

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PrimePCR™ SYBR® Green Assay: PDGFRA, Human
platelet-derived growth factor receptor, alpha polypeptide

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0007202
Info:   Same primer pair as used in probe assay qHsaCIP0026381
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PrimePCR™ Probe Assay: PDGFRA, Human
platelet-derived growth factor receptor, alpha polypeptide

Assay Type: Probe
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCIP0026381
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PrimePCR™ ddPCR™ Copy Number Assay: PDGFRA, Human
platelet-derived growth factor receptor, alpha polypeptide

Assay Type: Probe
Application: Copy Number Variation
Unique Assay ID: dHsaCP1000047
Info:   73nt amplicon; intronic
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:PDGFRA p.N659K, Human
platelet-derived growth factor receptor, alpha polypeptide

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2501760
Info:   58nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:PDGFRA WT for p.N659K, Human
platelet-derived growth factor receptor, alpha polypeptide

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2501761
Info:   58nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:PDGFRA p.T674I, Human
platelet-derived growth factor receptor, alpha polypeptide

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2502422
Info:   69nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:PDGFRA WT for p.T674I, Human
platelet-derived growth factor receptor, alpha polypeptide

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2502423
Info:   69nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:PDGFRA p.V824V, Human
platelet-derived growth factor receptor, alpha polypeptide

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2502956
Info:   65nt amplicon; exonic; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:PDGFRA WT for p.V824V, Human
platelet-derived growth factor receptor, alpha polypeptide

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2502957
Info:   65nt amplicon; exonic; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:PDGFRA p.D1071N, Human
platelet-derived growth factor receptor, alpha polypeptide

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2504102
Info:   65nt amplicon; exonic; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:PDGFRA WT for p.D1071N, Human
platelet-derived growth factor receptor, alpha polypeptide

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2504103
Info:   65nt amplicon; exonic; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:PDGFRA p.N659K, Human
platelet-derived growth factor receptor, alpha polypeptide

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2504730
Info:   58nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:PDGFRA WT for p.N659K, Human
platelet-derived growth factor receptor, alpha polypeptide

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2504731
Info:   58nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:PDGFRA p.V561D, Human
platelet-derived growth factor receptor, alpha polypeptide

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2504824
Info:   78nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:PDGFRA WT for p.V561D, Human
platelet-derived growth factor receptor, alpha polypeptide

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2504825
Info:   78nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   HEX
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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