Gene:  PCK1, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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This gene is a main control point for the regulation of gluconeogenesis. The cytosolic enzyme encoded by this gene along with GTP catalyzes the formation of phosphoenolpyruvate from oxaloacetate with the release of carbon dioxide and GDP. The expression of this gene can be regulated by insulin glucocorticoids glucagon cAMP and diet. Defects in this gene are a cause of cytosolic phosphoenolpyruvate carboxykinase deficiency. A mitochondrial isozyme of the encoded protein also has been characterized. [provided by RefSeq Jul 2008]

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PrimePCR™ SYBR® Green Assay: PCK1, Human
phosphoenolpyruvate carboxykinase 1 (soluble)

Assay Type: SYBR® Green
Assay Design: Exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0002640
Info:   Same primer pair as used in probe assay qHsaCEP0024455
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PrimePCR™ Probe Assay: PCK1, Human
phosphoenolpyruvate carboxykinase 1 (soluble)

Assay Type: Probe
Assay Design: Exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0024455
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PrimePCR™ ddPCR™ Copy Number Assay: PCK1, Human
phosphoenolpyruvate carboxykinase 1 (soluble)

Assay Type: Probe
Application: Copy Number Variation
Unique Assay ID: dHsaCP1000450
Info:   70nt amplicon; intronic
 
Fluorophore:   FAM
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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