Gene:  PAFAH1B1, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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This locus was identified as encoding a gene that when mutated or lost caused the lissencephaly associated with Miller-Dieker lissencephaly syndrome. This gene encodes the non-catalytic alpha subunit of the intracellular Ib isoform of platelet-activating factor acteylhydrolase a heterotrimeric enzyme that specifically catalyzes the removal of the acetyl group at the SN-2 position of platelet-activating factor (identified as 1-O-alkyl-2-acetyl-sn-glyceryl-3-phosphorylcholine). Two other isoforms of intracellular platelet-activating factor acetylhydrolase exist: one composed of multiple subunits the other a single subunit. In addition a single-subunit isoform of this enzyme is found in serum. [provided by RefSeq Apr 2009]

PrimePCR™ SYBR® Green Assay: PAFAH1B1, Human
platelet-activating factor acetylhydrolase 1b, regulatory subunit 1 (45kDa)

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0041933
Info:   Same primer pair as used in probe assay qHsaCEP0049196
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PrimePCR™ SYBR® Green Assay: PAFAH1B1, Human
platelet-activating factor acetylhydrolase 1b, regulatory subunit 1 (45kDa)

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0016656
Info:   Recommended - best efficiency
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PrimePCR™ Probe Assay: PAFAH1B1, Human
platelet-activating factor acetylhydrolase 1b, regulatory subunit 1 (45kDa)

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0049196
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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