Gene:  NRAS, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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This is an N-ras oncogene encoding a membrane protein that shuttles between the Golgi apparatus and the plasma membrane. This shuttling is regulated through palmitoylation and depalmitoylation by the ZDHHC9-GOLGA7 complex. The encoded protein which has intrinsic GTPase activity is activated by a guanine nucleotide-exchange factor and inactivated by a GTPase activating protein. Mutations in this gene have been associated with somatic rectal cancer follicular thyroid cancer autoimmune lymphoproliferative syndrome Noonan syndrome and juvenile myelomonocytic leukemia. [provided by RefSeq Jun 2011]

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PrimePCR™ SYBR® Green Assay: NRAS, Human
neuroblastoma RAS viral (v-ras) oncogene homolog

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0011527
Info:   Same primer pair as used in probe assay qHsaCIP0028063
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PrimePCR™ Probe Assay: NRAS, Human
neuroblastoma RAS viral (v-ras) oncogene homolog

Assay Type: Probe
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCIP0028063
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PrimePCR™ ddPCR™ Copy Number Assay: NRAS, Human
neuroblastoma RAS viral (v-ras) oncogene homolog

Assay Type: Probe
Application: Copy Number Variation
Unique Assay ID: dHsaCP1000493
Info:   66nt amplicon; exonic
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay: NRAS p.Q61H, Human
neuroblastoma RAS viral (v-ras) oncogene homolog

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2000065
Info:   65nt amplicon; exonic
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay: NRAS WT for p.Q61H, Human
neuroblastoma RAS viral (v-ras) oncogene homolog

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2000066
Info:   65nt amplicon; exonic
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay: NRAS p.Q61K, Human
neuroblastoma RAS viral (v-ras) oncogene homolog

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2000067
Info:   65nt amplicon; exonic
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay: NRAS WT for p.Q61K, Human
neuroblastoma RAS viral (v-ras) oncogene homolog

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2000068
Info:   65nt amplicon; exonic
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay: NRAS p.Q61L, Human
neuroblastoma RAS viral (v-ras) oncogene homolog

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2000069
Info:   65nt amplicon; exonic
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay: NRAS WT for p.Q61L, Human
neuroblastoma RAS viral (v-ras) oncogene homolog

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2000070
Info:   65nt amplicon; exonic
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay: NRAS p.Q61R, Human
neuroblastoma RAS viral (v-ras) oncogene homolog

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2000071
Info:   65nt amplicon; exonic
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay: NRAS WT for p.Q61R, Human
neuroblastoma RAS viral (v-ras) oncogene homolog

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2000072
Info:   65nt amplicon; exonic
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay: NRAS p.G12S, Human
neuroblastoma RAS viral (v-ras) oncogene homolog

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2000093
Info:   70nt amplicon; exonic
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay: NRAS WT for p.G12S, Human
neuroblastoma RAS viral (v-ras) oncogene homolog

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2000094
Info:   70nt amplicon; exonic
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay: NRAS p.G12D, Human
neuroblastoma RAS viral (v-ras) oncogene homolog

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2000095
Info:   70nt amplicon; exonic
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay: NRAS WT for p.G12D, Human
neuroblastoma RAS viral (v-ras) oncogene homolog

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2000096
Info:   70nt amplicon; exonic
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay: NRAS p.G13V, Human
neuroblastoma RAS viral (v-ras) oncogene homolog

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2500524
Info:   70nt amplicon; exonic
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay: NRAS WT for p.G13V, Human
neuroblastoma RAS viral (v-ras) oncogene homolog

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2500525
Info:   70nt amplicon; exonic
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay: NRAS p.G13D, Human
neuroblastoma RAS viral (v-ras) oncogene homolog

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2500526
Info:   70nt amplicon; exonic
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay: NRAS WT for p.G13D, Human
neuroblastoma RAS viral (v-ras) oncogene homolog

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2500527
Info:   70nt amplicon; exonic
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay: NRAS p.G12V, Human
neuroblastoma RAS viral (v-ras) oncogene homolog

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2500528
Info:   70nt amplicon; exonic
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay: NRAS WT for p.G12V, Human
neuroblastoma RAS viral (v-ras) oncogene homolog

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2500529
Info:   70nt amplicon; exonic
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay: NRAS p.G12C, Human
neuroblastoma RAS viral (v-ras) oncogene homolog

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2500530
Info:   70nt amplicon; exonic
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay: NRAS WT for p.G12C, Human
neuroblastoma RAS viral (v-ras) oncogene homolog

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2500531
Info:   70nt amplicon; exonic
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay: NRAS p.G13R, Human
neuroblastoma RAS viral (v-ras) oncogene homolog

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2500534
Info:   70nt amplicon; exonic
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay: NRAS WT for p.G13R, Human
neuroblastoma RAS viral (v-ras) oncogene homolog

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2500535
Info:   70nt amplicon; exonic
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay: NRAS p.G12R, Human
neuroblastoma RAS viral (v-ras) oncogene homolog

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2500560
Info:   70nt amplicon; exonic
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay: NRAS WT for p.G12R, Human
neuroblastoma RAS viral (v-ras) oncogene homolog

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2500561
Info:   70nt amplicon; exonic
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay: NRAS p.Q61H, Human
neuroblastoma RAS viral (v-ras) oncogene homolog

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2500578
Info:   65nt amplicon; exonic
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay: NRAS WT for p.Q61H, Human
neuroblastoma RAS viral (v-ras) oncogene homolog

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2500579
Info:   65nt amplicon; exonic
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:NRAS p.G13A, Human
neuroblastoma RAS viral (v-ras) oncogene homolog

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2501072
Info:   70nt amplicon; exonic; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:NRAS WT for p.G13A, Human
neuroblastoma RAS viral (v-ras) oncogene homolog

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2501073
Info:   70nt amplicon; exonic; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:NRAS p.Q61E, Human
neuroblastoma RAS viral (v-ras) oncogene homolog

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2501090
Info:   65nt amplicon; exonic; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:NRAS WT for p.Q61E, Human
neuroblastoma RAS viral (v-ras) oncogene homolog

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2501091
Info:   65nt amplicon; exonic; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:NRAS p.G13S, Human
neuroblastoma RAS viral (v-ras) oncogene homolog

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2501852
Info:   70nt amplicon; exonic; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:NRAS WT for p.G13S, Human
neuroblastoma RAS viral (v-ras) oncogene homolog

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2501853
Info:   70nt amplicon; exonic; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:NRAS p.G13C, Human
neuroblastoma RAS viral (v-ras) oncogene homolog

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2502500
Info:   70nt amplicon; exonic; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:NRAS WT for p.G13C, Human
neuroblastoma RAS viral (v-ras) oncogene homolog

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2502501
Info:   70nt amplicon; exonic; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:NRAS p.Q61P, Human
neuroblastoma RAS viral (v-ras) oncogene homolog

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2503018
Info:   65nt amplicon; exonic; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:NRAS WT for p.Q61P, Human
neuroblastoma RAS viral (v-ras) oncogene homolog

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2503019
Info:   65nt amplicon; exonic; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:NRAS p.Q61Q, Human
neuroblastoma RAS viral (v-ras) oncogene homolog

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2504622
Info:   65nt amplicon; exonic; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:NRAS WT for p.Q61Q, Human
neuroblastoma RAS viral (v-ras) oncogene homolog

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2504623
Info:   65nt amplicon; exonic; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:NRAS p.R68T, Human
neuroblastoma RAS viral (v-ras) oncogene homolog

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2504858
Info:   100nt amplicon; exonic; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:NRAS WT for p.R68T, Human
neuroblastoma RAS viral (v-ras) oncogene homolog

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2504859
Info:   100nt amplicon; exonic; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:NRAS p.A18T, Human
neuroblastoma RAS viral (v-ras) oncogene homolog

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2505740
Info:   65nt amplicon; exonic; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:NRAS WT for p.A18T, Human
neuroblastoma RAS viral (v-ras) oncogene homolog

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2505741
Info:   65nt amplicon; exonic; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:NRAS p.G60E, Human
neuroblastoma RAS viral (v-ras) oncogene homolog

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2505958
Info:   67nt amplicon; exonic; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:NRAS WT for p.G60E, Human
neuroblastoma RAS viral (v-ras) oncogene homolog

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2505959
Info:   67nt amplicon; exonic; in-silico
 
Fluorophore:   HEX
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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