Gene:  NRAS, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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This is an N-ras oncogene encoding a membrane protein that shuttles between the Golgi apparatus and the plasma membrane. This shuttling is regulated through palmitoylation and depalmitoylation by the ZDHHC9-GOLGA7 complex. The encoded protein which has intrinsic GTPase activity is activated by a guanine nucleotide-exchange factor and inactivated by a GTPase activating protein. Mutations in this gene have been associated with somatic rectal cancer follicular thyroid cancer autoimmune lymphoproliferative syndrome Noonan syndrome and juvenile myelomonocytic leukemia. [provided by RefSeq Jun 2011]

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PrimePCR™ SYBR® Green Assay: NRAS, Human
neuroblastoma RAS viral (v-ras) oncogene homolog

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0011527
Info:   Same primer pair as used in probe assay qHsaCIP0028063
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PrimePCR™ Probe Assay: NRAS, Human
neuroblastoma RAS viral (v-ras) oncogene homolog

Assay Type: Probe
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCIP0028063
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PrimePCR™ ddPCR™ Copy Number Assay: NRAS, Human
neuroblastoma RAS viral (v-ras) oncogene homolog

Assay Type: Probe
Application: Copy Number Variation
Unique Assay ID: dHsaCP1000493
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay: NRAS p.Q61H, Human
neuroblastoma RAS viral (v-ras) oncogene homolog

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2000065
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay: NRAS WT for p.Q61H, Human
neuroblastoma RAS viral (v-ras) oncogene homolog

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2000066
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay: NRAS p.Q61K, Human
neuroblastoma RAS viral (v-ras) oncogene homolog

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2000067
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay: NRAS WT for p.Q61K, Human
neuroblastoma RAS viral (v-ras) oncogene homolog

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2000068
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay: NRAS p.Q61L, Human
neuroblastoma RAS viral (v-ras) oncogene homolog

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2000069
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay: NRAS WT for p.Q61L, Human
neuroblastoma RAS viral (v-ras) oncogene homolog

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2000070
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay: NRAS p.Q61R, Human
neuroblastoma RAS viral (v-ras) oncogene homolog

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2000071
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay: NRAS WT for p.Q61R, Human
neuroblastoma RAS viral (v-ras) oncogene homolog

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2000072
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay: NRAS p.G12S, Human
neuroblastoma RAS viral (v-ras) oncogene homolog

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2000093
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay: NRAS WT for p.G12S, Human
neuroblastoma RAS viral (v-ras) oncogene homolog

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2000094
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay: NRAS p.G12D, Human
neuroblastoma RAS viral (v-ras) oncogene homolog

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2000095
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay: NRAS WT for p.G12D, Human
neuroblastoma RAS viral (v-ras) oncogene homolog

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2000096
 
Fluorophore:   HEX
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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