Gene:  NR3C1, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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This gene encodes glucocorticoid receptor which can function both as a transcription factor that binds to glucocorticoid response elements in the promoters of glucocorticoid responsive genes to activate their transcription and as a regulator of other transcription factors. This receptor is typically found in the cytoplasm but upon ligand binding is transported into the nucleus. It is involved in inflammatory responses cellular proliferation and differentiation in target tissues. Mutations in this gene are associated with generalized glucocorticoid resistance. Alternative splicing of this gene results in transcript variants encoding either the same or different isoforms. Additional isoforms resulting from the use of alternate in-frame translation initiation sites have also been described and shown to be functional displaying diverse cytoplasm-to-nucleus trafficking patterns and distinct transcriptional activities (PMID:15866175). [provided by RefSeq Feb 2011]

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PrimePCR™ SYBR® Green Assay: NR3C1, Human
nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor)

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0043506
Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0050768
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PrimePCR™ SYBR® Green Assay: NR3C1, Human
nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor)

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0023212
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PrimePCR™ Probe Assay: NR3C1, Human
nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor)

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0050768
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PrimePCR™ ddPCR™ Mutation Assay:NR3C1 p.L773P, Human
nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor)

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2502214
Info:   80nt amplicon; exonic; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:NR3C1 WT for p.L773P, Human
nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor)

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2502215
Info:   80nt amplicon; exonic; in-silico
 
Fluorophore:   HEX
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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