Gene:  NOTCH2, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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This gene encodes a member of the Notch family. Members of this Type 1 transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats and an intracellular domain consisting of multiple different domain types. Notch family members play a role in a variety of developmental processes by controlling cell fate decisions. The Notch signaling network is an evolutionarily conserved intercellular signaling pathway which regulates interactions between physically adjacent cells. In Drosophilia notch interaction with its cell-bound ligands (delta serrate) establishes an intercellular signaling pathway that plays a key role in development. Homologues of the notch-ligands have also been identified in human but precise interactions between these ligands and the human notch homologues remain to be determined. This protein is cleaved in the trans-Golgi network and presented on the cell surface as a heterodimer. This protein functions as a receptor for membrane bound ligands and may play a role in vascular renal and hepatic development. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Jan 2011]

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PrimePCR™ SYBR® Green Assay: NOTCH2, Human
notch 2

Assay Type: SYBR® Green
Assay Design: Exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0005739
Info:   Recommended - best specificity
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PrimePCR™ SYBR® Green Assay: NOTCH2, Human
notch 2

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0044544
Info:   Same primer pair as used in probe assay qHsaCEP0051806
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PrimePCR™ Probe Assay: NOTCH2, Human
notch 2

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0051806
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PrimePCR™ ddPCR™ Copy Number Assay: NOTCH2, Human
notch 2

Assay Type: Probe
Application: Copy Number Variation
Unique Assay ID: dHsaCP1000518
Info:   68nt amplicon; crosses exon-intron junction
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:NOTCH2 p.R2400*, Human
notch 2

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2502540
Info:   64nt amplicon; exonic; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:NOTCH2 WT for p.R2400*, Human
notch 2

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2502541
Info:   64nt amplicon; exonic; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:NOTCH2 p.V2075M, Human
notch 2

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2502650
Info:   65nt amplicon; exonic; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:NOTCH2 WT for p.V2075M, Human
notch 2

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2502651
Info:   65nt amplicon; exonic; in-silico
 
Fluorophore:   HEX
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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