Gene:  NOTCH1, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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This gene encodes a member of the Notch family. Members of this Type 1 transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats and an intracellular domain consisting of multiple different domain types. Notch family members play a role in a variety of developmental processes by controlling cell fate decisions. The Notch signaling network is an evolutionarily conserved intercellular signaling pathway which regulates interactions between physically adjacent cells. In Drosophilia notch interaction with its cell-bound ligands (delta serrate) establishes an intercellular signaling pathway that plays a key role in development. Homologues of the notch-ligands have also been identified in human but precise interactions between these ligands and the human notch homologues remain to be determined. This protein is cleaved in the trans-Golgi network and presented on the cell surface as a heterodimer. This protein functions as a receptor for membrane bound ligands and may play multiple roles during development. [provided by RefSeq Jul 2008]

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PrimePCR™ SYBR® Green Assay: NOTCH1, Human
notch 1

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0011825
Info:   Same primer pair as used in probe assay qHsaCIP0028194
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PrimePCR™ Probe Assay: NOTCH1, Human
notch 1

Assay Type: Probe
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCIP0028194
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PrimePCR™ ddPCR™ Copy Number Assay: NOTCH1, Human
notch 1

Assay Type: Probe
Application: Copy Number Variation
Unique Assay ID: dHsaCP1000509
Info:   58nt amplicon; exonic
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay: NOTCH1 p.P2514fs*4, Human
notch 1

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2500500
Info:   65nt amplicon; exonic
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay: NOTCH1 WT for p.P2514fs*4, Human
notch 1

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2500501
Info:   65nt amplicon; exonic
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:NOTCH1 p.L1600P, Human
notch 1

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2502004
Info:   65nt amplicon; exonic; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:NOTCH1 WT for p.L1600P, Human
notch 1

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2502005
Info:   65nt amplicon; exonic; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:NOTCH1 p.D1698D, Human
notch 1

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2502724
Info:   64nt amplicon; exonic; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:NOTCH1 WT for p.D1698D, Human
notch 1

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2502725
Info:   64nt amplicon; exonic; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:NOTCH1 p.Q2440*, Human
notch 1

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2502800
Info:   80nt amplicon; exonic; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:NOTCH1 WT for p.Q2440*, Human
notch 1

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2502801
Info:   80nt amplicon; exonic; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:NOTCH1 p.R1598P, Human
notch 1

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2503078
Info:   65nt amplicon; exonic; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:NOTCH1 WT for p.R1598P, Human
notch 1

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2503079
Info:   65nt amplicon; exonic; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:NOTCH1 p.I1616N, Human
notch 1

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2503316
Info:   56nt amplicon; exonic; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:NOTCH1 WT for p.I1616N, Human
notch 1

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2503317
Info:   56nt amplicon; exonic; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:NOTCH1 p.I1675I, Human
notch 1

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2503588
Info:   60nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:NOTCH1 WT for p.I1675I, Human
notch 1

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2503589
Info:   60nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:NOTCH1 p.R2327W, Human
notch 1

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2504290
Info:   59nt amplicon; exonic; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:NOTCH1 WT for p.R2327W, Human
notch 1

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2504291
Info:   59nt amplicon; exonic; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:NOTCH1 p.L1678P, Human
notch 1

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2505176
Info:   65nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:NOTCH1 WT for p.L1678P, Human
notch 1

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2505177
Info:   65nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:NOTCH1 p.L1600Q, Human
notch 1

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2505784
Info:   65nt amplicon; exonic; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:NOTCH1 WT for p.L1600Q, Human
notch 1

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2505785
Info:   65nt amplicon; exonic; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:NOTCH1 p.I1680N, Human
notch 1

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2505944
Info:   70nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:NOTCH1 WT for p.I1680N, Human
notch 1

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2505945
Info:   70nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   HEX
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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