Gene:  NFE2L2, Human

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This gene encodes a transcription factor which is a member of a small family of basic leucine zipper (bZIP) proteins. The encoded transcription factor regulates genes which contain antioxidant response elements (ARE) in their promoters; many of these genes encode proteins involved in response to injury and inflammation which includes the production of free radicals. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Jan 2012]

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PrimePCR™ SYBR® Green Assay: NFE2L2, Human
nuclear factor (erythroid-derived 2)-like 2

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0038543
Info:   Same primer pair as used in probe assay qHsaCEP0041265
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PrimePCR™ Probe Assay: NFE2L2, Human
nuclear factor (erythroid-derived 2)-like 2

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0041265
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PrimePCR™ ddPCR™ Mutation Assay:NFE2L2 p.G81V, Human
nuclear factor (erythroid-derived 2)-like 2

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2500904
Info:   68nt amplicon; exonic; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:NFE2L2 WT for p.G81V, Human
nuclear factor (erythroid-derived 2)-like 2

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2500905
Info:   68nt amplicon; exonic; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:NFE2L2 p.D29Y, Human
nuclear factor (erythroid-derived 2)-like 2

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2501184
Info:   78nt amplicon; exonic; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:NFE2L2 WT for p.D29Y, Human
nuclear factor (erythroid-derived 2)-like 2

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2501185
Info:   78nt amplicon; exonic; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:NFE2L2 p.L30F, Human
nuclear factor (erythroid-derived 2)-like 2

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2501238
Info:   79nt amplicon; exonic; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:NFE2L2 WT for p.L30F, Human
nuclear factor (erythroid-derived 2)-like 2

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2501239
Info:   79nt amplicon; exonic; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:NFE2L2 p.W24C, Human
nuclear factor (erythroid-derived 2)-like 2

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2501396
Info:   76nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:NFE2L2 WT for p.W24C, Human
nuclear factor (erythroid-derived 2)-like 2

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2501397
Info:   76nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:NFE2L2 p.G31A, Human
nuclear factor (erythroid-derived 2)-like 2

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2501570
Info:   80nt amplicon; exonic; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:NFE2L2 WT for p.G31A, Human
nuclear factor (erythroid-derived 2)-like 2

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2501571
Info:   80nt amplicon; exonic; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:NFE2L2 p.L30R, Human
nuclear factor (erythroid-derived 2)-like 2

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2501672
Info:   77nt amplicon; exonic; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:NFE2L2 WT for p.L30R, Human
nuclear factor (erythroid-derived 2)-like 2

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2501673
Info:   77nt amplicon; exonic; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:NFE2L2 p.E82Q, Human
nuclear factor (erythroid-derived 2)-like 2

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2502578
Info:   68nt amplicon; exonic; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:NFE2L2 WT for p.E82Q, Human
nuclear factor (erythroid-derived 2)-like 2

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2502579
Info:   68nt amplicon; exonic; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:NFE2L2 p.R34Q, Human
nuclear factor (erythroid-derived 2)-like 2

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2503042
Info:   64nt amplicon; exonic; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:NFE2L2 WT for p.R34Q, Human
nuclear factor (erythroid-derived 2)-like 2

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2503043
Info:   64nt amplicon; exonic; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:NFE2L2 p.R34G, Human
nuclear factor (erythroid-derived 2)-like 2

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2503462
Info:   64nt amplicon; exonic; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:NFE2L2 WT for p.R34G, Human
nuclear factor (erythroid-derived 2)-like 2

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2503463
Info:   64nt amplicon; exonic; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:NFE2L2 p.E82D, Human
nuclear factor (erythroid-derived 2)-like 2

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2503564
Info:   68nt amplicon; exonic; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:NFE2L2 WT for p.E82D, Human
nuclear factor (erythroid-derived 2)-like 2

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2503565
Info:   68nt amplicon; exonic; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:NFE2L2 p.E82G, Human
nuclear factor (erythroid-derived 2)-like 2

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2503822
Info:   68nt amplicon; exonic; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:NFE2L2 WT for p.E82G, Human
nuclear factor (erythroid-derived 2)-like 2

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2503823
Info:   68nt amplicon; exonic; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:NFE2L2 p.G81D, Human
nuclear factor (erythroid-derived 2)-like 2

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2504752
Info:   68nt amplicon; exonic; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:NFE2L2 WT for p.G81D, Human
nuclear factor (erythroid-derived 2)-like 2

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2504753
Info:   68nt amplicon; exonic; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:NFE2L2 p.D29H, Human
nuclear factor (erythroid-derived 2)-like 2

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2505138
Info:   78nt amplicon; exonic; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:NFE2L2 WT for p.D29H, Human
nuclear factor (erythroid-derived 2)-like 2

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2505139
Info:   78nt amplicon; exonic; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:NFE2L2 p.T80K, Human
nuclear factor (erythroid-derived 2)-like 2

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2505628
Info:   68nt amplicon; exonic; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:NFE2L2 WT for p.T80K, Human
nuclear factor (erythroid-derived 2)-like 2

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2505629
Info:   68nt amplicon; exonic; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:NFE2L2 p.D29G, Human
nuclear factor (erythroid-derived 2)-like 2

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2505732
Info:   78nt amplicon; exonic; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:NFE2L2 WT for p.D29G, Human
nuclear factor (erythroid-derived 2)-like 2

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2505733
Info:   78nt amplicon; exonic; in-silico designed
 
Fluorophore:   HEX
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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