Gene:  NF1, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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This gene product appears to function as a negative regulator of the ras signal transduction pathway. Mutations in this gene have been linked to neurofibromatosis type 1 juvenile myelomonocytic leukemia and Watson syndrome. The mRNA for this gene is subject to RNA editing (CGA>UGA->Arg1306Term) resulting in premature translation termination. Alternatively spliced transcript variants encoding different isoforms have also been described for this gene. [provided by RefSeq Jul 2008]

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PrimePCR™ SYBR® Green Assay: NF1, Human
neurofibromin 1

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0017889
Info:   Same primer pair as used in probe assay qHsaCIP0031526
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PrimePCR™ Probe Assay: NF1, Human
neurofibromin 1

Assay Type: Probe
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCIP0031526
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PrimePCR™ ddPCR™ Copy Number Assay: NF1, Human
neurofibromin 1

Assay Type: Probe
Application: Copy Number Variation
Unique Assay ID: dHsaCP1000177
Info:   70nt amplicon; exonic
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:NF1 p.R440*, Human
neurofibromin 1

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2501474
Info:   69nt amplicon; exonic; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:NF1 WT for p.R440*, Human
neurofibromin 1

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2501475
Info:   69nt amplicon; exonic; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:NF1 p.R1769*, Human
neurofibromin 1

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2501792
Info:   65nt amplicon; exonic; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:NF1 WT for p.R1769*, Human
neurofibromin 1

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2501793
Info:   65nt amplicon; exonic; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:NF1 p.R2150R, Human
neurofibromin 1

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2502080
Info:   98nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:NF1 WT for p.R2150R, Human
neurofibromin 1

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2502081
Info:   98nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:NF1 p.R461*, Human
neurofibromin 1

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2502992
Info:   70nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:NF1 WT for p.R461*, Human
neurofibromin 1

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2502993
Info:   70nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:NF1 p.M645V, Human
neurofibromin 1

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2503002
Info:   74nt amplicon; exonic; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:NF1 WT for p.M645V, Human
neurofibromin 1

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2503003
Info:   74nt amplicon; exonic; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:NF1 p.R1362*, Human
neurofibromin 1

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2503196
Info:   76nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:NF1 WT for p.R1362*, Human
neurofibromin 1

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2503197
Info:   76nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:NF1 p.P2472H, Human
neurofibromin 1

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2503220
Info:   77nt amplicon; exonic; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:NF1 WT for p.P2472H, Human
neurofibromin 1

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2503221
Info:   77nt amplicon; exonic; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:NF1 p.R2450*, Human
neurofibromin 1

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2503464
Info:   79nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:NF1 WT for p.R2450*, Human
neurofibromin 1

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2503465
Info:   79nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:NF1 p.P1951L, Human
neurofibromin 1

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2504882
Info:   65nt amplicon; exonic; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:NF1 WT for p.P1951L, Human
neurofibromin 1

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2504883
Info:   65nt amplicon; exonic; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:NF1 p.L1880L, Human
neurofibromin 1

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2505142
Info:   66nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:NF1 WT for p.L1880L, Human
neurofibromin 1

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2505143
Info:   66nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   HEX
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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