Gene:  MTOR, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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The protein encoded by this gene belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. The ANGPTL7 gene is located in an intron of this gene. [provided by RefSeq Sep 2008]

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PrimePCR™ SYBR® Green Assay: MTOR, Human
mechanistic target of rapamycin (serine/threonine kinase)

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0048371
Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0055631
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PrimePCR™ SYBR® Green Assay: MTOR, Human
mechanistic target of rapamycin (serine/threonine kinase)

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0012480
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PrimePCR™ Probe Assay: MTOR, Human
mechanistic target of rapamycin (serine/threonine kinase)

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0055631
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: MTOR, Human
mechanistic target of rapamycin (serine/threonine kinase)

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5012607
Info:   EG; Same primer pair as used in qPCR assay qHsaCED0048371; exonic
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: MTOR, Human
mechanistic target of rapamycin (serine/threonine kinase)

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5018663
Info:   EG; Same primer pair as used in qPCR assay qHsaCID0012480; Intron-spanning
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PrimePCR™ ddPCR™ Expression Probe Assay: MTOR, Human
mechanistic target of rapamycin (serine/threonine kinase)

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5043524
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCEP0055631; exonic
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Expression Probe Assay: MTOR, Human
mechanistic target of rapamycin (serine/threonine kinase)

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5043525
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCEP0055631; exonic
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:MTOR p.E1799K, Human
mechanistic target of rapamycin (serine/threonine kinase)

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2503594
Info:   61nt amplicon; exonic; in silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:MTOR WT for p.E1799K, Human
mechanistic target of rapamycin (serine/threonine kinase)

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2503595
Info:   61nt amplicon; exonic; in silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:MTOR p.S2215Y, Human
mechanistic target of rapamycin (serine/threonine kinase)

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2504998
Info:   65nt amplicon; crosses exon-intron junction; in silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:MTOR WT for p.S2215Y, Human
mechanistic target of rapamycin (serine/threonine kinase)

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2504999
Info:   65nt amplicon; crosses exon-intron junction; in silico
 
Fluorophore:   HEX
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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