Gene:  Mthfd1, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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This gene encodes a trifunctional cytoplasmic enzyme. The encoded protein functions as a methylenetetrahydrofolate dehydrogenase a methenyltetrahydrofolate cyclohydrolase and a formyltetrahydrofolate synthase. The encoded enzyme functions in de novo synthesis of purines and thymidylate and in regeneration of methionine from homocysteine. [provided by RefSeq Oct 2009]

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PrimePCR™ SYBR® Green Assay: Mthfd1, Rat
C-1-tetrahydrofolate synthase, cytoplasmic

Assay Type: SYBR® Green
Assay Design: intron_spanning
Application: Gene Expression
Unique Assay ID: qRnoCID0002354
List Price:    $134.00
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PrimePCR™ Probe Assay: Mthfd1, Rat
C-1-tetrahydrofolate synthase, cytoplasmic

Assay Type: Probe
Assay Design: intron_spanning
Application: Gene Expression
Unique Assay ID: qRnoCIP0024185
List Price:    $196.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: Mthfd1, Rat
C-1-tetrahydrofolate synthase, cytoplasmic

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dRnoEG5136339
Info:   EG; Same primer pair as used in qPCR assay qRnoCID0002354; intron_spanning
List Price:    $134.00
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PrimePCR™ ddPCR™ Expression Probe Assay: Mthfd1, Rat
C-1-tetrahydrofolate synthase, cytoplasmic

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dRnoCPE5168656
Info:   FAM; Same primer pair and probe as used in qPCR assay qRnoCIP0024185; intron_spanning
 
Fluorophore:   FAM
List Price:    $201.00
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PrimePCR™ ddPCR™ Expression Probe Assay: Mthfd1, Rat
C-1-tetrahydrofolate synthase, cytoplasmic

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dRnoCPE5168657
Info:   HEX; Same primer pair and probe as used in qPCR assay qRnoCIP0024185; intron_spanning
 
Fluorophore:   HEX
List Price:    $201.00
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    Related pathways not available
Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download