Gene:  MSH2, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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MSH2 was identified as a locus frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). When cloned it was discovered to be a human homolog of the E. coli mismatch repair gene mutS consistent with the characteristic alterations in microsatellite sequences (RER+ phenotype) found in HNPCC. [provided by RefSeq Jul 2008]

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PrimePCR™ SYBR® Green Assay: MSH2, Human
mutS homolog 2, colon cancer, nonpolyposis type 1 (E. coli)

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0042676
Info:   Same primer pair as used in probe assay qHsaCEP0049938
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PrimePCR™ SYBR® Green Assay: MSH2, Human
mutS homolog 2, colon cancer, nonpolyposis type 1 (E. coli)

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0012204
Info:   Recommended - best coverage
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PrimePCR™ Probe Assay: MSH2, Human
mutS homolog 2, colon cancer, nonpolyposis type 1 (E. coli)

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0049938
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PrimePCR™ ddPCR™ Copy Number Assay: MSH2, Human
mutS homolog 2, colon cancer, nonpolyposis type 1 (E. coli)

Assay Type: Probe
Application: Copy Number Variation
Unique Assay ID: dHsaCP2500431
Info:   70nt amplicon; intronic
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:MSH2 p.P202P, Human
mutS homolog 2, colon cancer, nonpolyposis type 1 (E. coli)

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2501836
Info:   84nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:MSH2 WT for p.P202P, Human
mutS homolog 2, colon cancer, nonpolyposis type 1 (E. coli)

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2501837
Info:   84nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:MSH2 p.R711*, Human
mutS homolog 2, colon cancer, nonpolyposis type 1 (E. coli)

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2502006
Info:   76nt amplicon; exonic; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:MSH2 WT for p.R711*, Human
mutS homolog 2, colon cancer, nonpolyposis type 1 (E. coli)

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2502007
Info:   76nt amplicon; exonic; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:MSH2 p.R406R, Human
mutS homolog 2, colon cancer, nonpolyposis type 1 (E. coli)

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2502204
Info:   76nt amplicon; exonic; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:MSH2 WT for p.R406R, Human
mutS homolog 2, colon cancer, nonpolyposis type 1 (E. coli)

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2502205
Info:   76nt amplicon; exonic; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:MSH2 p.R680*, Human
mutS homolog 2, colon cancer, nonpolyposis type 1 (E. coli)

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2503930
Info:   68nt amplicon; exonic; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:MSH2 WT for p.R680*, Human
mutS homolog 2, colon cancer, nonpolyposis type 1 (E. coli)

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2503931
Info:   68nt amplicon; exonic; in-silico
 
Fluorophore:   HEX
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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