Gene:  MLH1, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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This gene was identified as a locus frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). It is a human homolog of the E. coli DNA mismatch repair gene mutL consistent with the characteristic alterations in microsatellite sequences (RER+phenotype) found in HNPCC. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional transcript variants have been described but their full-length natures have not been determined.[provided by RefSeq Nov 2009]

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PrimePCR™ SYBR® Green Assay: MLH1, Human
mutL homolog 1, colon cancer, nonpolyposis type 2 (E. coli)

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0042382
Info:   Same primer pair as used in probe assay qHsaCEP0049644
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PrimePCR™ SYBR® Green Assay: MLH1, Human
mutL homolog 1, colon cancer, nonpolyposis type 2 (E. coli)

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0012582
Info:   Recommended - best coverage
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PrimePCR™ Probe Assay: MLH1, Human
mutL homolog 1, colon cancer, nonpolyposis type 2 (E. coli)

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0049644
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PrimePCR™ ddPCR™ Mutation Assay:MLH1 p.M587V, Human
mutL homolog 1, colon cancer, nonpolyposis type 2 (E. coli)

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2502954
Info:   65nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:MLH1 WT for p.M587V, Human
mutL homolog 1, colon cancer, nonpolyposis type 2 (E. coli)

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2502955
Info:   65nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:MLH1 p.R522W, Human
mutL homolog 1, colon cancer, nonpolyposis type 2 (E. coli)

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2504662
Info:   93nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:MLH1 WT for p.R522W, Human
mutL homolog 1, colon cancer, nonpolyposis type 2 (E. coli)

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2504663
Info:   93nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:MLH1 p.R226Q, Human
mutL homolog 1, colon cancer, nonpolyposis type 2 (E. coli)

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2505852
Info:   100nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:MLH1 WT for p.R226Q, Human
mutL homolog 1, colon cancer, nonpolyposis type 2 (E. coli)

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2505853
Info:   100nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   HEX
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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