Gene:  MET, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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The proto-oncogene MET product is the hepatocyte growth factor receptor and encodes tyrosine-kinase activity. The primary single chain precursor protein is post-translationally cleaved to produce the alpha and beta subunits which are disulfide linked to form the mature receptor. Various mutations in the MET gene are associated with papillary renal carcinoma. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Jul 2008]

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PrimePCR™ SYBR® Green Assay: MET, Human
met proto-oncogene (hepatocyte growth factor receptor)

Assay Type: SYBR® Green
Assay Design: Exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0002004
Info:   Recommended - best coverage
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PrimePCR™ SYBR® Green Assay: MET, Human
met proto-oncogene (hepatocyte growth factor receptor)

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0043116
Info:   Same primer pair as used in probe assay qHsaCEP0050378
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PrimePCR™ Probe Assay: MET, Human
met proto-oncogene (hepatocyte growth factor receptor)

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0050378
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: MET, Human
met proto-oncogene (hepatocyte growth factor receptor)

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5000806
Info:   EG; Same primer pair as used in qPCR assay qHsaCED0002004; Exonic
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: MET, Human
met proto-oncogene (hepatocyte growth factor receptor)

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5007930
Info:   EG; Same primer pair as used in qPCR assay qHsaCED0043116; exonic
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PrimePCR™ ddPCR™ Copy Number Assay: MET, Human
met proto-oncogene (hepatocyte growth factor receptor)

Assay Type: Probe
Application: Copy Number Variation
Unique Assay ID: dHsaCP1000038
Info:   FAM: 109nt amplicon; exonic
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Copy Number Assay: MET, Human
met proto-oncogene (hepatocyte growth factor receptor)

Assay Type: Probe
Application: Copy Number Variation
Unique Assay ID: dHsaCP2500321
Info:   FAM; 62nt amplicon; exonic; FAM version of MET (dHsaCP2506521)
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Copy Number Assay: MET, Human
met proto-oncogene (hepatocyte growth factor receptor)

Assay Type: Probe
Application: Copy Number Variation
Unique Assay ID: dHsaCP2506521
Info:   HEX; 62nt amplicon; exonic; HEX version of MET (dHsaCP2500321)
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay: MET p.Y1253D, Human
met proto-oncogene (hepatocyte growth factor receptor)

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2506840
Info:   78nt amplicon; exonic
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay: MET WT for p.Y1253D, Human
met proto-oncogene (hepatocyte growth factor receptor)

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2506841
Info:   78nt amplicon; exonic
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Expression Probe Assay: MET, Human
met proto-oncogene (hepatocyte growth factor receptor)

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5034172
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCEP0050378; exonic
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Expression Probe Assay: MET, Human
met proto-oncogene (hepatocyte growth factor receptor)

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5034173
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCEP0050378; exonic
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:MET p.M1268T, Human
met proto-oncogene (hepatocyte growth factor receptor)

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2502350
Info:   70nt amplicon; exonic; in silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:MET WT for p.M1268T, Human
met proto-oncogene (hepatocyte growth factor receptor)

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2502351
Info:   70nt amplicon; exonic; in silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:MET p.T1010I, Human
met proto-oncogene (hepatocyte growth factor receptor)

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2503878
Info:   70nt amplicon; exonic; in silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:MET WT for p.T1010I, Human
met proto-oncogene (hepatocyte growth factor receptor)

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2503879
Info:   70nt amplicon; exonic; in silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:MET p.Y1253D, Human
met proto-oncogene (hepatocyte growth factor receptor)

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2505024
Info:   78nt amplicon; exonic; in silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:MET WT for p.Y1253D, Human
met proto-oncogene (hepatocyte growth factor receptor)

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2505025
Info:   78nt amplicon; exonic; in silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:MET p.Y1248C, Human
met proto-oncogene (hepatocyte growth factor receptor)

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2506040
Info:   79nt amplicon; exonic; in silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:MET WT for p.Y1248C, Human
met proto-oncogene (hepatocyte growth factor receptor)

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2506041
Info:   79nt amplicon; exonic; in silico
 
Fluorophore:   HEX
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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