Gene:  MDM2, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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This gene is a target gene of the transcription factor tumor protein p53. The encoded protein is a nuclear phosphoprotein that binds and inhibits transactivation by tumor protein p53 as part of an autoregulatory negative feedback loop. Overexpression of this gene can result in excessive inactivation of tumor protein p53 diminishing its tumor suppressor function. This protein has E3 ubiquitin ligase activity which targets tumor protein p53 for proteasomal degradation. This protein also affects the cell cycle apoptosis and tumorigenesis through interactions with other proteins including retinoblastoma 1 and ribosomal protein L5. More than 40 different alternatively spliced transcript variants have been isolated from both tumor and normal tissues. [provided by RefSeq Jul 2008]

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PrimePCR™ SYBR® Green Assay: MDM2, Human
Mdm2 p53 binding protein homolog (mouse)

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0044623
Info:   Same primer pair as used in probe assay qHsaCEP0051885
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PrimePCR™ SYBR® Green Assay: MDM2, Human
Mdm2 p53 binding protein homolog (mouse)

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0011000
Info:   Recommended - best coverage
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PrimePCR™ Probe Assay: MDM2, Human
Mdm2 p53 binding protein homolog (mouse)

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0051885
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PrimePCR™ ddPCR™ Copy Number Assay: MDM2, Human
Mdm2 p53 binding protein homolog (mouse)

Assay Type: Probe
Application: Copy Number Variation
Unique Assay ID: dHsaCP1000036
Info:   94nt amplicon; exonic
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Copy Number Assay: MDM2, Human
Mdm2 p53 binding protein homolog (mouse)

Assay Type: Probe
Application: Copy Number Variation
Unique Assay ID: dHsaCP2500317
Info:   70nt amplicon; crosses exon-intron junction
 
Fluorophore:   FAM
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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