Gene:  Mdc1, Mouse

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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The protein encoded by this gene contains an N-terminal forkhead domain two BRCA1 C-terminal (BRCT) motifs and a central domain with 7 divergent copies of an approximately 41-amino acid sequence. The encoded protein is required to activate the intra-S phase and G2/M phase cell cycle checkpoints in response to DNA damage. This nuclear protein interacts with phosphorylated histone H2AX near sites of DNA double-strand breaks through its BRCT motifs and facilitates recruitment of the ATM kinase and meiotic recombination 11 protein complex to DNA damage foci. Mice with mutations in this gene exhibit growth retardation male infertility immune defects chromosome instability DNA repair defects and radiation sensitivity. [provided by RefSeq Jul 2008]

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PrimePCR™ SYBR® Green Assay: Mdc1, Mouse
Mediator of DNA damage checkpoint 1

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qMmuCED0051642
Info:   Same primer pair as used in probe assay qMmuCEP0060068
List Price:    $125.00
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PrimePCR™ SYBR® Green Assay: Mdc1, Mouse
Mediator of DNA damage checkpoint 1

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qMmuCID0005891
List Price:    $125.00
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PrimePCR™ Probe Assay: Mdc1, Mouse
Mediator of DNA damage checkpoint 1

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qMmuCEP0060068
List Price:    $180.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: Mdc1, Mouse
Mediator of DNA damage checkpoint 1

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dMmuEG5071492
Info:   EG; Same primer pair as used in qPCR assay qMmuCED0051642; exonic
List Price:    $125.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: Mdc1, Mouse
Mediator of DNA damage checkpoint 1

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dMmuEG5073202
Info:   EG; Same primer pair as used in qPCR assay qMmuCID0005891; Intron-spanning
List Price:    $125.00
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PrimePCR™ ddPCR™ Expression Probe Assay: Mdc1, Mouse
Mediator of DNA damage checkpoint 1

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dMmuCPE5106614
Info:   FAM; Same primer pair and probe as used in qPCR assay qMmuCEP0060068; exonic
 
Fluorophore:   FAM
List Price:    $190.00
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PrimePCR™ ddPCR™ Expression Probe Assay: Mdc1, Mouse
Mediator of DNA damage checkpoint 1

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dMmuCPE5106615
Info:   HEX; Same primer pair and probe as used in qPCR assay qMmuCEP0060068; exonic
 
Fluorophore:   HEX
List Price:    $190.00
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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