Gene:  MDC1, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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Design and Validation of Real-Time PCR Primers
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The protein encoded by this gene contains an N-terminal forkhead domain two BRCA1 C-terminal (BRCT) motifs and a central domain with 13 repetitions of an approximately 41-amino acid sequence. The encoded protein is required to activate the intra-S phase and G2/M phase cell cycle checkpoints in response to DNA damage. This nuclear protein interacts with phosphorylated histone H2AX near sites of DNA double-strand breaks through its BRCT motifs and facilitates recruitment of the ATM kinase and meiotic recombination 11 protein complex to DNA damage foci. [provided by RefSeq Jul 2008]

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PrimePCR™ SYBR® Green Assay: MDC1, Human
mediator of DNA-damage checkpoint 1

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0037094
Info:   Same primer pair as used in probe assay qHsaCEP0039817
List Price:    $125.00
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PrimePCR™ Probe Assay: MDC1, Human
mediator of DNA-damage checkpoint 1

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0039817
List Price:    $180.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: MDC1, Human
mediator of DNA-damage checkpoint 1

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5188319
Info:   EG; Same primer pair as used in qPCR assay qHsaCED0037094; exonic
List Price:    $125.00
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PrimePCR™ ddPCR™ Expression Probe Assay: MDC1, Human
mediator of DNA-damage checkpoint 1

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5190329
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCEP0039817; exonic
 
Fluorophore:   FAM
List Price:    $190.00
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PrimePCR™ ddPCR™ Expression Probe Assay: MDC1, Human
mediator of DNA-damage checkpoint 1

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5190330
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCEP0039817; exonic
 
Fluorophore:   HEX
List Price:    $190.00
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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