Gene:  MBD4, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2 MBD1 MBD2 MBD3 and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins with the exception of MBD3 is capable of binding specifically to methylated DNA. MBD4 may function to mediate the biological consequences of the methylation signal. In addition MBD4 has protein sequence similarity to bacterial DNA repair enzymes and thus may have some function in DNA repair. Further MBD4 gene mutations are detected in tumors with primary microsatellite-instability (MSI) a form of genomic instability associated with defective DNA mismatch repair and MBD4 gene meets 4 of 5 criteria of a bona fide MIS target gene. [provided by RefSeq Jul 2008]

Select a product type :   All   qPCR   ddPCR
PrimePCR™ SYBR® Green Assay: MBD4, Human
methyl-CpG binding domain protein 4

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0044247
Info:   Same primer pair as used in probe assay qHsaCEP0051509
List Price:    $130.00
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PrimePCR™ SYBR® Green Assay: MBD4, Human
methyl-CpG binding domain protein 4

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0010609
List Price:    $130.00
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PrimePCR™ Probe Assay: MBD4, Human
methyl-CpG binding domain protein 4

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0051509
List Price:    $190.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: MBD4, Human
methyl-CpG binding domain protein 4

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5008921
Info:   EG; Same primer pair as used in qPCR assay qHsaCED0044247; exonic
List Price:    $130.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: MBD4, Human
methyl-CpG binding domain protein 4

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5017245
Info:   EG; Same primer pair as used in qPCR assay qHsaCID0010609; Intron-spanning
List Price:    $130.00
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PrimePCR™ ddPCR™ Expression Probe Assay: MBD4, Human
methyl-CpG binding domain protein 4

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5036154
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCEP0051509; exonic
 
Fluorophore:   FAM
List Price:    $195.00
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PrimePCR™ ddPCR™ Expression Probe Assay: MBD4, Human
methyl-CpG binding domain protein 4

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5036155
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCEP0051509; exonic
 
Fluorophore:   HEX
List Price:    $195.00
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    Related pathways not available
Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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