Gene:  MAP3K5, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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Mitogen-activated protein kinase (MAPK) signaling cascades include MAPK or extracellular signal-regulated kinase (ERK) MAPK kinase (MKK or MEK) and MAPK kinase kinase (MAPKKK or MEKK). MAPKK kinase/MEKK phosphorylates and activates its downstream protein kinase MAPK kinase/MEK which in turn activates MAPK. The kinases of these signaling cascades are highly conserved and homologs exist in yeast Drosophila and mammalian cells. MAPKKK5 contains 1374 amino acids with all 11 kinase subdomains. Northern blot analysis shows that MAPKKK5 transcript is abundantly expressed in human heart and pancreas. The MAPKKK5 protein phosphorylates and activates MKK4 (aliases SERK1 MAPKK4) in vitro and activates c-Jun N-terminal kinase (JNK)/stress-activated protein kinase (SAPK) during transient expression in COS and 293 cells; MAPKKK5 does not activate MAPK/ERK. [provided by RefSeq Jul 2008]

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PrimePCR™ SYBR® Green Assay: MAP3K5, Human
mitogen-activated protein kinase kinase kinase 5

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0016590
Info:   Same primer pair as used in probe assay qHsaCIP0030685
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PrimePCR™ Probe Assay: MAP3K5, Human
mitogen-activated protein kinase kinase kinase 5

Assay Type: Probe
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCIP0030685
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PrimePCR™ ddPCR™ Copy Number Assay: MAP3K5, Human
mitogen-activated protein kinase kinase kinase 5

Assay Type: Probe
Application: Copy Number Variation
Unique Assay ID: dHsaCP1000311
 
Fluorophore:   FAM
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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