Gene:  LGMN, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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This gene encodes a cysteine protease that has a strict specificity for hydrolysis of asparaginyl bonds. This enzyme may be involved in the processing of bacterial peptides and endogenous proteins for MHC class II presentation in the lysosomal/endosomal systems. Enzyme activation is triggered by acidic pH and appears to be autocatalytic. Protein expression occurs after monocytes differentiate into dendritic cells. A fully mature active enzyme is produced following lipopolysaccharide expression in mature dendritic cells. Overexpression of this gene may be associated with the majority of solid tumor types. This gene has a pseudogene on chromosome 13. Several alternatively spliced transcript variants have been described but the biological validity of only two has been determined. These two variants encode the same isoform. [provided by RefSeq Jul 2008]

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PrimePCR™ SYBR® Green Assay: LGMN, Human
legumain

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0042807
Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0050069
List Price:    $138.00
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PrimePCR™ SYBR® Green Assay: LGMN, Human
legumain

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0012935
List Price:    $138.00
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PrimePCR™ Probe Assay: LGMN, Human
legumain

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0050069
List Price:    $202.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: LGMN, Human
legumain

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5018992
Info:   EG; Same primer pair as used in qPCR assay qHsaCID0012935; Intron-spanning
List Price:    $138.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: LGMN, Human
legumain

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5189051
Info:   EG; Same primer pair as used in qPCR assay qHsaCED0042807; exonic
List Price:    $138.00
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PrimePCR™ ddPCR™ Expression Probe Assay: LGMN, Human
legumain

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5191793
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCEP0050069; exonic
 
Fluorophore:   FAM
List Price:    $207.00
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PrimePCR™ ddPCR™ Expression Probe Assay: LGMN, Human
legumain

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5191794
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCEP0050069; exonic
 
Fluorophore:   HEX
List Price:    $207.00
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