Gene:  LFNG, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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This gene is a member of the fringe gene family which also includes radical and manic fringe genes. They all encode evolutionarily conserved glycosyltransferases that act in the Notch signaling pathway to define boundaries during embryonic development. While their genomic structure is distinct from other glycosyltransferases fringe proteins have a fucose-specific beta-13-N-acetylglucosaminyltransferase activity that leads to elongation of O-linked fucose residues on Notch which alters Notch signaling. This gene product is predicted to be a single-pass type II Golgi membrane protein but it may also be secreted and proteolytically processed like the related proteins in mouse and Drosophila (PMID: 9187150). Mutations in this gene have been associated with autosomal recessive spondylocostal dysostosis 3. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Oct 2009]

PrimePCR™ SYBR® Green Assay: LFNG, Human
LFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0043121
Info:   Same primer pair as used in probe assay qHsaCEP0050383
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PrimePCR™ SYBR® Green Assay: LFNG, Human
LFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0014069
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PrimePCR™ Probe Assay: LFNG, Human
LFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0050383
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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