Gene:  LDHA, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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The protein encoded by this gene catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. The protein is found predominantly in muscle tissue and belongs to the lactate dehydrogenase family. Mutations in this gene have been linked to exertional myoglobinuria. Multiple transcript variants encoding different isoforms have been found for this gene. The human genome contains several non-transcribed pseudogenes of this gene. [provided by RefSeq Sep 2008]

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PrimePCR™ SYBR® Green Assay: LDHA, Human
lactate dehydrogenase A

Assay Type: SYBR® Green
Assay Design: Exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0001212
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PrimePCR™ SYBR® Green Assay: LDHA, Human
lactate dehydrogenase A

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0056429
Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0057534
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PrimePCR™ Probe Assay: LDHA, Human
lactate dehydrogenase A

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0057534
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PrimePCR™ ddPCR™ Mutation Assay:LDHA p.V209A, Human
lactate dehydrogenase A

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2505580
Info:   80nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:LDHA WT for p.V209A, Human
lactate dehydrogenase A

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2505581
Info:   80nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   HEX
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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