Gene:  KRAS, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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This gene a Kirsten ras oncogene homolog from the mammalian ras gene family encodes a protein that is a member of the small GTPase superfamily. A single amino acid substitution is responsible for an activating mutation. The transforming protein that results is implicated in various malignancies including lung adenocarcinoma mucinous adenoma ductal carcinoma of the pancreas and colorectal carcinoma. Alternative splicing leads to variants encoding two isoforms that differ in the C-terminal region. [provided by RefSeq Jul 2008]

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PrimePCR™ SYBR® Green Assay: KRAS, Human
v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0038999
Info:   Same primer pair as used in probe assay qHsaCEP0041721
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PrimePCR™ Probe Assay: KRAS, Human
v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0041721
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PrimePCR™ ddPCR™ Copy Number Assay: KRAS, Human
v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog

Assay Type: Probe
Application: Copy Number Variation
Unique Assay ID: dHsaCP1000033
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay: KRAS p.G12D, Human
v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2000001
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay: KRAS WT for p.G12D, Human
v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2000002
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay: KRAS p.G12A, Human
v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2000003
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay: KRAS WT for p.G12A, Human
v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2000004
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay: KRAS p.G12V, Human
v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2000005
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay: KRAS WT for p.G12V, Human
v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2000006
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay: KRAS p.G12C, Human
v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2000007
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay: KRAS WT for p.G12C, Human
v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2000008
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay: KRAS p.G12R, Human
v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2000009
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay: KRAS WT for p.G12R, Human
v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2000010
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay: KRAS p.G12S, Human
v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2000011
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay: KRAS WT for p.G12S, Human
v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2000012
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay: KRAS p.G13D, Human
v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2000013
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay: KRAS WT for p.G13D, Human
v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2000014
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay: KRAS p.A146T, Human
v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2000079
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay: KRAS WT for p.A146T, Human
v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2000080
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay: KRAS p.Q61L, Human
v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2000101
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay: KRAS WT for p.Q61L, Human
v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2000102
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay: KRAS p.G13C, Human
v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2000113
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay: KRAS WT for p.G13C, Human
v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2000114
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay: KRAS p.Q61H, Human
v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2000131
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay: KRAS WT for p.Q61H, Human
v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2000132
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay: KRAS p.Q61H, Human
v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2000133
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay: KRAS WT for p.Q61H, Human
v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2000134
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay: KRAS p.Q61R, Human
v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2000135
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay: KRAS WT for p.Q61R, Human
v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2000136
 
Fluorophore:   HEX
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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