Gene:  ITGAV, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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ITAGV encodes integrin alpha chain V. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. The I-domain containing integrin alpha V undergoes post-translational cleavage to yield disulfide-linked heavy and light chains that combine with multiple integrin beta chains to form different integrins. Among the known associating beta chains (beta chains 1356 and 8; 'ITGB1' 'ITGB3' 'ITGB5' 'ITGB6' and 'ITGB8') each can interact with extracellular matrix ligands; the alpha V beta 3 integrin perhaps the most studied of these is referred to as the Vitronectin receptor (VNR). In addition to adhesion many integrins are known to facilitate signal transduction. Alternative splicing results in multiple transcript variants. [provided by RefSeq Feb 2009]

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PrimePCR™ SYBR® Green Assay: ITGAV, Human
integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51)

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0006233
Info:   Same primer pair as used in probe assay qHsaCIP0026077
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PrimePCR™ Probe Assay: ITGAV, Human
integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51)

Assay Type: Probe
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCIP0026077
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PrimePCR™ ddPCR™ Mutation Assay:ITGAV p.R775K, Human
integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51)

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2500854
Info:   77nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:ITGAV WT for p.R775K, Human
integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51)

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2500855
Info:   77nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:ITGAV p.L23I, Human
integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51)

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2505674
Info:   63nt amplicon; exonic; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:ITGAV WT for p.L23I, Human
integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51)

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2505675
Info:   63nt amplicon; exonic; in-silico
 
Fluorophore:   HEX
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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