Gene:  IL7R, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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The protein encoded by this gene is a receptor for interleukine 7 (IL7). The function of this receptor requires the interleukin 2 receptor gamma chain (IL2RG) which is a common gamma chain shared by the receptors of various cytokines including interleukine 2 4 7 9 and 15. This protein has been shown to play a critical role in the V(D)J recombination during lymphocyte development. This protein is also found to control the accessibility of the TCR gamma locus by STAT5 and histone acetylation. Knockout studies in mice suggested that blocking apoptosis is an essential function of this protein during differentiation and activation of T lymphocytes. The functional defects in this protein may be associated with the pathogenesis of the severe combined immunodeficiency (SCID). [provided by RefSeq Jul 2008]

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PrimePCR™ SYBR® Green Assay: IL7R, Human
interleukin 7 receptor

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0056514
Info:   Same primer pair as used in probe assay qHsaCEP0057619
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PrimePCR™ SYBR® Green Assay: IL7R, Human
interleukin 7 receptor

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0015056
Info:   Recommended - best coverage
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PrimePCR™ Probe Assay: IL7R, Human
interleukin 7 receptor

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0057619
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PrimePCR™ ddPCR™ Mutation Assay:IL7R p.T56A, Human
interleukin 7 receptor

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2504022
Info:   65nt amplicon; exonic; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:IL7R WT for p.T56A, Human
interleukin 7 receptor

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2504023
Info:   65nt amplicon; exonic; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:IL7R p.S185C, Human
interleukin 7 receptor

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2504876
Info:   68nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:IL7R WT for p.S185C, Human
interleukin 7 receptor

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2504877
Info:   68nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   HEX
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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