Gene:  IL12A, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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This gene encodes a subunit of a cytokine that acts on T and natural killer cells and has a broad array of biological activities. The cytokine is a disulfide-linked heterodimer composed of the 35-kD subunit encoded by this gene and a 40-kD subunit that is a member of the cytokine receptor family. This cytokine is required for the T-cell-independent induction of interferon (IFN)-gamma and is important for the differentiation of both Th1 and Th2 cells. The responses of lymphocytes to this cytokine are mediated by the activator of transcription protein STAT4. Nitric oxide synthase 2A (NOS2A/NOS2) is found to be required for the signaling process of this cytokine in innate immunity. [provided by RefSeq Jul 2008]

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PrimePCR™ SYBR® Green Assay: IL12A, Human
interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35)

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0046580
Info:   Same primer pair as used in probe assay qHsaCEP0053841
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PrimePCR™ SYBR® Green Assay: IL12A, Human
interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35)

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0007212
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PrimePCR™ Probe Assay: IL12A, Human
interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35)

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0053841
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: IL12A, Human
interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35)

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5011011
Info:   EG; Same primer pair as used in qPCR assay qHsaCED0046580; exonic
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: IL12A, Human
interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35)

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5014904
Info:   EG; Same primer pair as used in qPCR assay qHsaCID0007212; Intron-spanning
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PrimePCR™ ddPCR™ Expression Probe Assay: IL12A, Human
interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35)

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5040332
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCEP0053841; exonic
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Expression Probe Assay: IL12A, Human
interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35)

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5040333
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCEP0053841; exonic
 
Fluorophore:   HEX
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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