Gene:  IDH1, Human

print
PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

Why PrimePCR?

PrimePCR
Lookup Tool
Design and Validation of Real-Time PCR Primers
Pathway Curation
Control and Reference Assays for Real-Time PCR

Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases which localize to the mitochondrial matrix and two NADP(+)-dependent isocitrate dehydrogenases one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions such as the conversion of 2 4-dienoyl-CoAs to 3-enoyl-CoAs as well as in peroxisomal reactions that consume 2-oxoglutarate namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production. [provided by RefSeq Jul 2008]

Select a product type :   All   qPCR   ddPCR
PrimePCR™ SYBR® Green Assay: IDH1, Human
isocitrate dehydrogenase 1 (NADP+), soluble

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0044840
Info:   Same primer pair as used in probe assay qHsaCEP0052102
List Price:   
Your Price:   Log In
Quantity:  
 
Add to Custom Plate
PrimePCR™ SYBR® Green Assay: IDH1, Human
isocitrate dehydrogenase 1 (NADP+), soluble

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0012601
List Price:   
Your Price:   Log In
Quantity:  
 
Add to Custom Plate
PrimePCR™ Probe Assay: IDH1, Human
isocitrate dehydrogenase 1 (NADP+), soluble

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0052102
List Price:   
Your Price:   Log In
Quantity:  
 
PrimePCR™ ddPCR™ Copy Number Assay: IDH1, Human
isocitrate dehydrogenase 1 (NADP+), soluble

Assay Type: Probe
Application: Copy Number Variation
Unique Assay ID: dHsaCP1000498
Info:   70nt amplicon; exonic
 
Fluorophore:   FAM
List Price:   
Your Price:   Log In
Quantity:  
 
PrimePCR™ ddPCR™ Mutation Assay: IDH1 p.R132C, Human
isocitrate dehydrogenase 1 (NADP+), soluble

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2000053
Info:   63nt amplicon; crosses exon-intron junction
 
Fluorophore:   FAM
List Price:   
Your Price:   Log In
Quantity:  
 
PrimePCR™ ddPCR™ Mutation Assay: IDH1 WT for p.R132C, Human
isocitrate dehydrogenase 1 (NADP+), soluble

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2000054
Info:   63nt amplicon; crosses exon-intron junction
 
Fluorophore:   HEX
List Price:   
Your Price:   Log In
Quantity:  
 
PrimePCR™ ddPCR™ Mutation Assay: IDH1 p.R132H, Human
isocitrate dehydrogenase 1 (NADP+), soluble

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2000055
Info:   63nt amplicon; crosses exon-intron junction
 
Fluorophore:   FAM
List Price:   
Your Price:   Log In
Quantity:  
 
PrimePCR™ ddPCR™ Mutation Assay: IDH1 WT for p.R132H, Human
isocitrate dehydrogenase 1 (NADP+), soluble

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2000056
Info:   63nt amplicon; crosses exon-intron junction
 
Fluorophore:   HEX
List Price:   
Your Price:   Log In
Quantity:  
 
PrimePCR™ ddPCR™ Mutation Assay: IDH1 p.R132G, Human
isocitrate dehydrogenase 1 (NADP+), soluble

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2500512
Info:   78nt amplicon; crosses exon-intron junction
 
Fluorophore:   FAM
List Price:   
Your Price:   Log In
Quantity:  
 
PrimePCR™ ddPCR™ Mutation Assay: IDH1 WT for p.R132G, Human
isocitrate dehydrogenase 1 (NADP+), soluble

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2500513
Info:   78nt amplicon; crosses exon-intron junction
 
Fluorophore:   HEX
List Price:   
Your Price:   Log In
Quantity:  
 
PrimePCR™ ddPCR™ Mutation Assay:IDH1 p.V178I, Human
isocitrate dehydrogenase 1 (NADP+), soluble

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2501360
Info:   80nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   FAM
List Price:   
Your Price:   Log In
Quantity:  
 
PrimePCR™ ddPCR™ Mutation Assay:IDH1 WT for p.V178I, Human
isocitrate dehydrogenase 1 (NADP+), soluble

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2501361
Info:   80nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   HEX
List Price:   
Your Price:   Log In
Quantity:  
 
PrimePCR™ ddPCR™ Mutation Assay:IDH1 p.R132S, Human
isocitrate dehydrogenase 1 (NADP+), soluble

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2504792
Info:   78nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   FAM
List Price:   
Your Price:   Log In
Quantity:  
 
PrimePCR™ ddPCR™ Mutation Assay:IDH1 WT for p.R132S, Human
isocitrate dehydrogenase 1 (NADP+), soluble

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2504793
Info:   78nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   HEX
List Price:   
Your Price:   Log In
Quantity:  
 
PrimePCR™ ddPCR™ Mutation Assay:IDH1 p.R132L, Human
isocitrate dehydrogenase 1 (NADP+), soluble

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2505486
Info:   78nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   FAM
List Price:   
Your Price:   Log In
Quantity:  
 
PrimePCR™ ddPCR™ Mutation Assay:IDH1 WT for p.R132L, Human
isocitrate dehydrogenase 1 (NADP+), soluble

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2505487
Info:   78nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   HEX
List Price:   
Your Price:   Log In
Quantity:  
 
    Related pathways not available
Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
Get Help

Call us at 1-800-4-BIORAD (1-800-424-6723)

Sign Up for Bio-Rad Updates!
Enter your email address below to receive your choice of the latest news, promotions, and more.